ArticleNucleic acids research1989
Oligonucleotide-targeted degradation of U1 and U2 snRNAs reveals differential interactions of simian virus 40 pre-mRNAs with snRNPs.
Article in Nucleic acids research, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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Who cites it
8 citing papers in PubMed, 17 citations in OpenAlex.
- Genetics and biochemistry remain essential in the structural era of the spliceosome.Methods (San Diego, Calif.) · 2017Review
- Mitotic noncoding RNA processing promotes kinetochore and spindle assembly in Xenopus.The Journal of cell biology · 2016Article
- Splicing-independent recruitment of spliceosomal small nuclear RNPs to nascent RNA polymerase II transcripts.The Journal of cell biology · 2007Article
- Three small nucleolar RNAs that are involved in ribosomal RNA precursor processing.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Disruption of pre-mRNA splicing in vivo results in reorganization of splicing factors.The Journal of cell biology · 1994Article
- Pathways for selection of 5' splice sites by U1 snRNPs and SF2/ASF.The EMBO journal · 1993Article
- Localization of pre-messenger RNA at discrete nuclear sites.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
- Trans-activating rev protein of the human immunodeficiency virus 1 interacts directly and specifically with its target RNA.Proceedings of the National Academy of Sciences of the United States of America · 1990Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We have investigated the roles of U1 and U2 snRNP particles in SV40 pre-mRNA splicing by oligonucleotide-targeted degradation of U1 or U2 snRNAs in Xenopus laevis oocytes. Microinjection of oligonucleotides complementary to regions of U1 or U2 RNAs either in the presence or absence of SV40 DNA resulted in specific cleavage of the corresponding snRNA. Unexpectedly, degradation of U1 or U2 snRNA was far more extensive when the oligonucleotide was injected without, or prior to, introduction of viral DNA. In either co-injected or pre-injected oocytes, these oligonucleotides caused a dramatic reduction in the accumulation of spliced SV40 mRNA expressed from the viral late region, and a commensurate increase in unspliced late RNA. When pre-injected, two different U2 specific oligonucleotides also inhibited the formation of both large and small tumor antigen spliced early mRNAs. However, even when, by pre-injection of a U1 5' end-specific oligonucleotide, greater than 95% degradation of the U1 snRNA 5' ends occurred in oocytes, no reduction in early pre-mRNA splicing was observed. In contrast, the same U1 5' end oligonucleotide, when added to HeLa splicing extracts, substantially inhibited the splicing of SV40 early pre-mRNA, indicating that U1 mRNP is not totally dispensable for early splicing. These findings confirm and extend our earlier observations which suggested that different pre-mRNAs vary in their requirements for snRNPs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.