ArticleMolecular and cellular biology1989
Identification of four nuclear transport signal-binding proteins that interact with diverse transport signals.
Article in Molecular and cellular biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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43 citing papers in PubMed, 101 citations in OpenAlex.
- Characterization of a Drosophila phosphorylation-dependent nuclear-localization-signal-binding protein.The Biochemical journal · 1997Article
- The cell cycle-dependent nuclear import of v-Jun is regulated by phosphorylation of a serine adjacent to the nuclear localization signal.The Journal of cell biology · 1995Article
- Two nuclear localization signals present in the basic-helix 1 domains of MyoD promote its active nuclear translocation and can function independently.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Histone-poly(A) hybrid molecules as tools to block nuclear pores.Journal of protein chemistry · 1995Article
- The serum response factor nuclear localization signal: general implications for cyclic AMP-dependent protein kinase activity in control of nuclear translocation.Molecular and cellular biology · 1995Article
- Differential roles of heat shock protein 70 in the in vitro nuclear import of glucocorticoid receptor and simian virus 40 large tumor antigen.Molecular and cellular biology · 1994Article
- Nucleocytoplasmic transport.The Biochemical journal · 1994Review
- The maize abscisic acid-responsive protein Rab17 is located in the nucleus and interacts with nuclear localization signals.The Plant cell · 1994Article
- Eukaryotic initiation factor 5A is a cellular target of the human immunodeficiency virus type 1 Rev activation domain mediating trans-activation.The Journal of cell biology · 1993Article
- Specific binding of nuclear localization sequences to plant nuclei.The Plant cell · 1993Article
- Extragenic suppressors of mutations in the cytoplasmic C terminus of SEC63 define five genes in Saccharomyces cerevisiae.Genetics · 1993Article
- A cytoplasmically anchored nuclear protein interferes specifically with the import of nuclear proteins but not U1 snRNA.The Journal of cell biology · 1993Article
- Purification and characterization of a DNA-binding heterodimer of 52 and 100 kDa from HeLa cells.The Biochemical journal · 1993Article
- Role of nuclear pore complex in simian virus 40 nuclear targeting.Journal of virology · 1993Article
- Antibodies against 70-kD heat shock cognate protein inhibit mediated nuclear import of karyophilic proteins.The Journal of cell biology · 1992Article
- A new subclass of nucleoporins that functionally interact with nuclear pore protein NSP1.The EMBO journal · 1992Article
- Enhancement of polyhedrin nuclear localization during baculovirus infection.Journal of virology · 1992Article
- Cooperation of proto-signals for nuclear accumulation of estrogen and progesterone receptors.The EMBO journal · 1992Article
- Analysis of a developmentally regulated nuclear localization signal in Xenopus.The Journal of cell biology · 1992Article
- A mutant nuclear protein with similarity to RNA binding proteins interferes with nuclear import in yeast.Molecular biology of the cell · 1992Article
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3 authors at 1 institution in 1 country.
Funding
Abstract
The transport of proteins into the nucleus requires not only the presence of a nuclear transport signal on the targeted protein but also the signal recognition proteins and the nuclear pore translocation apparatus. Complicating the search for the signal recognition proteins is the fact that the nuclear transport signals identified share little obvious homology. In this study, synthetic peptides homologous to the nuclear transport signals from the simian virus 40 large T antigen, Xenopus oocyte nucleoplasmin, adenovirus E1A, and Saccharomyces cerevisiae MAT alpha 2 proteins were coupled to a UV-photoactivable cross-linker and iodinated for use in an in vitro cross-linking reaction with cellular lysates. Four proteins, p140, p100, p70, and p55, which specifically interacted with the nuclear transport signal peptides were identified. Unique patterns of reactivity were observed with closely related pairs of nuclear transport signal peptides. Competition experiments with labeled and unlabeled peptides demonstrated that heterologous signals were able to bind the same protein and suggested that diverse signals use a common transport pathway. The subcellular distribution of the four nuclear transport signal-binding proteins suggested that nuclear transport involves both cytoplasmic and nuclear receptors. The four proteins were not bound by wheat germ agglutinin and were not associated tightly with the nuclear pore complex.
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