ArticlePloS one2014
In vivo selection of resistant E. coli after ingestion of milk with added drug residues.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 63 citations in OpenAlex.
- Impacts of feeding preweaned calves milk containing drug residues on the functional profile of the fecal microbiota.Scientific reports · 2018Trial
- Camel-Associated Antimicrobial Resistance: An Overlooked One Health Interface.Veterinary sciences · 2026Review
- Epidemiology of Antimicrobial Residues and Phenotypic Resistance of Bacterial Isolates from Waste Milk on California Dairies.Microorganisms · 2026Article
- Evaluation of ß-lactamase-induced inactivation of penicillin G and cefoperazone in spiked ultra-high temperature and raw unpasteurized milk.Frontiers in veterinary science · 2026Article
- The future of udder health: Antimicrobial stewardship and alternative therapy of bovine mastitis.JDS communications · 2025Review
- Evaluating bacterial growth in raw, frozen, and heat-treated colostrum inoculated with fecalJDS communications · 2025Article
- Ciprofloxacin Concentrations in Food Could Select for Quinolone Resistance inAntibiotics (Basel, Switzerland) · 2024Article
- Efficacy of Copper Ion Treatment on Bacteria and Antibiotic Residues Contained in Bovine Waste Milk.Antibiotics (Basel, Switzerland) · 2024Article
- Extended-Spectrum Beta-Lactamase-ProducingAntibiotics (Basel, Switzerland) · 2024Review
- Antimicrobial Resistance Pattern ofAnimals : an open access journal from MDPI · 2022Article
- Analysis of Antimicrobial Use and the Presence of Antimicrobial-Resistant Bacteria on Austrian Dairy Farms-A Pilot Study.Antibiotics (Basel, Switzerland) · 2022Article
- An Overview of Waste Milk Feeding Effect on Growth Performance, Metabolism, Antioxidant Status and Immunity of Dairy Calves.Frontiers in veterinary science · 2022Review
- Identifying Associations in Minimum Inhibitory Concentration Values ofFrontiers in veterinary science · 2022Article
- Antimicrobial Resistance ofAnimals : an open access journal from MDPI · 2021Article
- Differences in the fecal microbiota of dairy calves reared with differing sources of milk and levels of maternal contact.JDS communications · 2021Article
- ESBL/AmpC-ProducingAnimals : an open access journal from MDPI · 2021Article
- Calf health from birth to weaning - an update.Irish veterinary journal · 2021Review
- Selection of Resistant Bacteria in Mallards Exposed to Subinhibitory Concentrations of Ciprofloxacin in Their Water Environment.Antimicrobial agents and chemotherapy · 2021Article
- The Effects of Feeding Waste Milk Containing Antimicrobial Residues on Dairy Calf Health.Pathogens (Basel, Switzerland) · 2021Review
- Survey on Antimicrobial Drug Use Practices in California Preweaned Dairy Calves.Frontiers in veterinary science · 2021Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Antimicrobial resistance represents a major global threat to modern medicine. In vitro studies have shown that very low concentrations of drugs, as frequently identified in the environment, and in foods and water for human and animal consumption, can select for resistant bacteria. However, limited information is currently available on the in vivo impact of ingested drug residues. The objective of our study was to evaluate the effect of feeding preweaned calves milk containing antimicrobial drug residues (below the minimum inhibitory concentration), similar to concentrations detected in milk commonly fed to dairy calves, on selection of resistant fecal E. coli in calves from birth to weaning. At birth, thirty calves were randomly assigned to a controlled feeding trial where: 15 calves were fed raw milk with no drug residues (NR), and 15 calves were fed raw milk with drug residues (DR) by adding ceftiofur, penicillin, ampicillin, and oxytetracycline at final concentrations in the milk of 0.1, 0.005, 0.01, and 0.3 µg/ml, respectively. Fecal samples were rectally collected from each calf once a week starting at birth prior to the first feeding in the trial (pre-treatment) until 6 weeks of age. A significantly greater proportion of E. coli resistant to ampicillin, cefoxitin, ceftiofur, streptomycin and tetracycline was observed in DR calves when compared to NR calves. Additionally, isolates from DR calves had a significant decrease in susceptibility to ceftriaxone and ceftiofur when compared to isolates from NR calves. A greater proportion of E. coli isolates from calves in the DR group were resistant to 3 or more antimicrobial drugs when compared to calves in the ND group. These findings highlight the role that low concentrations of antimicrobial drugs have on the evolution and selection of resistance to multiple antimicrobial drugs in vivo.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.