Evidence map›Paper›PMID 25506719›Full record

ArticleNature methods2015

Fast native-SAD phasing for routine macromolecular structure determination.

Tobias Weinert, Vincent Olieric, Sandro Waltersperger, Ezequiel Panepucci, Lirong Chen, Hua Zhang, Dayong Zhou, John Rose, Akio Ebihara, Seiki Kuramitsu and 18 more

Erratum issuedAbstract read
PubMed Publisher
In one paragraph

Article in Nature methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 79 papers.

0numbers the graph read from it
0cells of the map it votes in
79citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

79 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. X-Ray Crystallography for Macromolecular Complexes.Advances in experimental medicine and biology · 2024
    Article
  9. Article
  10. Article
  11. Article
  12. Review
  13. Article
  14. Article
  15. Article
  16. Article
  17. Review
  18. Article
  19. Article
  20. Native SAD phasing at room temperature.Acta crystallographica. Section D, Structural biology · 2022
    Article

19 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

  • Erratum issued
5 · Who and what money

Authors and funding

28 authors.

Tobias WeinertSwiss Light Source at Paul Scherrer Institut, Villigen, Switzerland.
Vincent OliericSwiss Light Source at Paul Scherrer Institut, Villigen, Switzerland.
Sandro WalterspergerSwiss Light Source at Paul Scherrer Institut, Villigen, Switzerland.
Ezequiel PanepucciSwiss Light Source at Paul Scherrer Institut, Villigen, Switzerland.
Lirong ChenDepartment of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA.
Hua ZhangDepartment of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA.
Dayong ZhouDepartment of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA.
John RoseDepartment of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA.
Akio EbiharaLaboratory of Biological Chemistry, Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan.
Seiki KuramitsuDepartment of Biological Sciences, Graduate School of Science, Osaka University, Osaka, Japan.
Dianfan LiMembrane Structural and Functional Biology Group, School of Medicine and School of Biochemistry and Immunology, Trinity College Dublin, Dublin, Ireland.
Nicole HoweMembrane Structural and Functional Biology Group, School of Medicine and School of Biochemistry and Immunology, Trinity College Dublin, Dublin, Ireland.
Gisela SchnappBoehringer Ingelheim Pharma GmbH and Co. KG, Biberach an der Riss, Germany.
Alexander PautschBoehringer Ingelheim Pharma GmbH and Co. KG, Biberach an der Riss, Germany.
Katja BargstenLaboratory of Biomolecular Research, Department of Biology and Chemistry, Paul Scherrer Institut, Villigen, Switzerland.
Andrea E ProtaLaboratory of Biomolecular Research, Department of Biology and Chemistry, Paul Scherrer Institut, Villigen, Switzerland.
Parag SuranaNational Centre for Biological Sciences, Bangalore, India.
Jithesh KotturNational Centre for Biological Sciences, Bangalore, India.
Deepak T NairNational Centre for Biological Sciences, Bangalore, India.
Federica BasilicoDepartment of Experimental Oncology, European Institute of Oncology, Milan, Italy.ORCID 0000000270026316
Valentina CecatielloDepartment of Experimental Oncology, European Institute of Oncology, Milan, Italy.
Sebastiano PasqualatoDepartment of Experimental Oncology, European Institute of Oncology, Milan, Italy.
Andreas BolandDepartment of Biochemistry, Max Planck Institute for Developmental Biology, Tübingen, Germany.
Oliver WeichenriederDepartment of Biochemistry, Max Planck Institute for Developmental Biology, Tübingen, Germany.
Bi-Cheng WangDepartment of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA.
Michel O SteinmetzLaboratory of Biomolecular Research, Department of Biology and Chemistry, Paul Scherrer Institut, Villigen, Switzerland.
Martin CaffreyMembrane Structural and Functional Biology Group, School of Medicine and School of Biochemistry and Immunology, Trinity College Dublin, Dublin, Ireland.
Meitian WangSwiss Light Source at Paul Scherrer Institut, Villigen, Switzerland.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We describe a data collection method that uses a single crystal to solve X-ray structures by native SAD (single-wavelength anomalous diffraction). We solved the structures of 11 real-life examples, including a human membrane protein, a protein-DNA complex and a 266-kDa multiprotein-ligand complex, using this method. The data collection strategy is suitable for routine structure determination and can be implemented at most macromolecular crystallography synchrotron beamlines.

Indexed as

AnimalsDNA-Binding ProteinsHumansMembrane ProteinsModels, MolecularMultiprotein ComplexesProtein ConformationSoftwareSynchrotronsX-Ray DiffractionDNA-Binding ProteinsMembrane ProteinsMultiprotein Complexes

Identifiers

PMID25506719

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.