ArticleBioMed research international2014
3D dynamic culture of rabbit articular chondrocytes encapsulated in alginate gel beads using spinner flasks for cartilage tissue regeneration.
Article in BioMed research international, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Co-culture engineering: a promising strategy for production of engineered extracellular vesicle for osteoarthritis treatment.Cell communication and signaling : CCS · 2024Review
- The Effects of Mechanical Load on Chondrogenic Responses of Bone Marrow Mesenchymal Stem Cells and Chondrocytes Encapsulated in Chondroitin Sulfate-Based Hydrogel.International journal of molecular sciences · 2023Article
- Multiple Cell Cultures for MRI Analysis.International journal of molecular sciences · 2022Review
- Article
- The Application of Bioreactors for Cartilage Tissue Engineering: Advances, Limitations, and Future Perspectives.Stem cells international · 2021Review
- Confocal scanning of intervertebral disc cells in 3D: Inside alginate beads and in native microenvironment.JOR spine · 2020Article
- Non-contact Coculture Reveals a Comprehensive Response of Chondrocytes Induced by Mesenchymal Stem Cells Through Trophic Secretion.Tissue engineering and regenerative medicine · 2018Article
- Phytoestrogen (Daidzein) Promotes Chondrogenic Phenotype of Human Chondrocytes in 2D and 3D Culture Systems.Tissue engineering and regenerative medicine · 2017Article
- Polycaprolactone Triol-Citrate Scaffolds Enriched with Human Platelet Releasates Promote Chondrogenic Phenotype and Cartilage Extracellular Matrix Formation.Tissue engineering and regenerative medicine · 2017Article
- Article
- Mesenchymal Stem Cells Reshape and Provoke Proliferation of Articular Chondrocytes by Paracrine Secretion.Scientific reports · 2016Article
- Poly(ε-caprolactone)-based substrates bearing pendant small chemical groups as a platform for systemic investigation of chondrogenesis.Cell proliferation · 2016Article
- Calcium/Cobalt Alginate Beads as Functional Scaffolds for Cartilage Tissue Engineering.Stem cells international · 2016Article
- Chitosan enriched three-dimensional matrix reduces inflammatory and catabolic mediators production by human chondrocytes.PloS one · 2015Article
Corrections and comments
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Authors and funding
6 authors.
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Abstract
Cell-based therapy using chondrocytes for cartilage repair suffers from chondrocyte dedifferentiation. In the present study, the effects of an integrated three-dimensional and dynamic culture on rabbit articular chondrocytes were investigated. Cells (passages 1 and 4) were encapsulated in alginate gel beads and cultured in spinner flasks in chondrogenic and chondrocyte growth media. Subcutaneous implantation of the cell-laden beads was performed to evaluate the ectopic chondrogenesis. It was found that cells remained viable after 35 days in the three-dimensional dynamic culture. Passage 1 cells demonstrated a proliferative growth in both media. Passage 4 cells showed a gradual reduction in DNA content in growth medium, which was attenuated in chondrogenic medium. Deposition of glycosaminoglycans (GAG) was found in all cultures. While passage 1 cells generally produced higher amounts of GAG than passage 4 cells, GAG/DNA became similar on day 35 for both cells in growth media. Interestingly, GAG/DNA in growth medium was greater than that in chondrogenic medium for both cells. Based on GAG quantification and gene expression analysis, encapsulated passage 1 cells cultured in growth medium displayed the best ectopic chondrogenesis. Taken together, the three-dimensional and dynamic culture for chondrocytes holds great potential in cartilage regeneration.
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