ArticleFrontiers in physics2014
Super-resolution imaging of C-type lectin spatial rearrangement within the dendritic cell plasma membrane at fungal microbe contact sites.
Article in Frontiers in physics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- Lipopolysaccharide insertion and vesicle mediated turnover drive growth independent outer membrane adaptation in Escherichia coli.Nature communications · 2026Article
- Propulsive cell entry diverts pathogens from immune degradation by remodeling the phagocytic synapse.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Dectin-1 multimerization and signaling depends on fungal β-glucan structure and exposure.Biophysical journal · 2023Article
- Nanoscale clustering of mycobacterial ligands and DC-SIGN host receptors are key determinants for pathogen recognition.Science advances · 2023Article
- Propulsive cell entry diverts pathogens from immune degradation by remodeling the phagocytic synapse.bioRxiv : the preprint server for biology · 2023Article
- Insights into dendritic cell maturation during infection with application of advanced imaging techniques.Frontiers in cellular and infection microbiology · 2023Review
- Toward a new picture of the living plasma membrane.Protein science : a publication of the Protein Society · 2020Review
- The Decade of Super-Resolution Microscopy of the Presynapse.Frontiers in synaptic neuroscience · 2020Review
- Phagocytosis is mediated by two-dimensional assemblies of the F-BAR protein GAS7.Nature communications · 2019Article
- Antigen structure affects cellular routing through DC-SIGN.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Review
- Visualisation and analysis of hepatitis C virus non-structural proteins using super-resolution microscopy.Scientific reports · 2018Article
- A computational model for regulation of nanoscale glucan exposure in Candida albicans.PloS one · 2017Article
- Inhomogeneity Based Characterization of Distribution Patterns on the Plasma Membrane.PLoS computational biology · 2016Article
- Systemic localization of seven major types of carbohydrates on cell membranes by dSTORM imaging.Scientific reports · 2016Article
- Complexity Revealed: A Hierarchy of Clustered Membrane Proteins.Biophysical journal · 2016Article
- Nanoscopic cell-wall architecture of an immunogenic ligand in Candida albicans during antifungal drug treatment.Molecular biology of the cell · 2016Article
- Effect of Spatial Inhomogeneities on the Membrane Surface on Receptor Dimerization and Signal Initiation.Frontiers in cell and developmental biology · 2016Article
- Dances with Membranes: Breakthroughs from Super-resolution Imaging.Current topics in membranes · 2015Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Dendritic cells express DC-SIGN and CD206, C-type lectins (CTLs) that bind a variety of pathogens and may facilitate pathogen uptake for subsequent antigen presentation. Both proteins form punctate membrane nanodomains (∼80 nm) on naïve cells. We analyzed the spatiotemporal distribution of CTLs following host-fungal particle contact using confocal microscopy and three distinct methods of cluster identification and measurement of receptor clusters in super-resolution datasets: DBSCAN, Pair Correlation and a custom implementation of the Getis spatial statistic. Quantitative analysis of confocal and super-resolution images demonstrated that CTL nanodomains become concentrated in the contact site relative to non-contact membrane after the first hour of exposure and established that this recruitment is sustained out to 4 h. DC-SIGN nanodomains in fungal contact sites exhibit a 70% area increase and a 38% decrease in interdomain separation. Contact site CD206 nanodomains possess 90% greater area and 42% lower interdomain separation relative to non-contact regions. Contact site CTL clusters appear as disk-shaped domains of approximately 150-175 nm in diameter. The increase in length scale of CTL nanostructure in contact sites suggests that the smaller nanodomains on resting membranes may merge during fungal recognition, or that they become packed closely enough to achieve sub-resolution inter-domain edge separations of <30 nm. This study provides evidence of local receptor spatial rearrangements on the nanoscale that occur in the plasma membrane upon pathogen binding and may direct important signaling interactions required to recognize and respond to the presence of a relatively large pathogen.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.