ArticleFrontiers in plant science2014
Transgenic plants expressing ω-ACTX-Hv1a and snowdrop lectin (GNA) fusion protein show enhanced resistance to aphids.
Article in Frontiers in plant science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 36 citations in OpenAlex.
- Fusion protein based strategies for developing insect-resistant crops.Functional & integrative genomics · 2026Review
- Structural insights into insect-selective sodium channel toxins drive AI-enhanced biopesticide design.Nature communications · 2026Article
- The fusion protein of scorpion neurotoxin BjαIT and Galanthus nivalis agglutinin (GNA) enhanced the injection insecticidal activity against silkworms, but only has lethal activity against newly hatched larva when administered orally.World journal of microbiology & biotechnology · 2024Article
- Coat protein of a whitefly-vectored plant virus as a delivery system to target whitefly.Microbial biotechnology · 2024Article
- Comparative Transcriptome Analysis Reveals the Molecular Basis ofPlants (Basel, Switzerland) · 2023Article
- Biotechnological Approaches for Host Plant Resistance to Insect Pests.Frontiers in genetics · 2022Review
- A sugarcane mosaic virus vector for rapid in planta screening of proteins that inhibit the growth of insect herbivores.Plant biotechnology journal · 2021Article
- Strategies for Heterologous Expression, Synthesis, and Purification of Animal Venom Toxins.Frontiers in bioengineering and biotechnology · 2021Review
- Genetic engineering of crops for insect resistance: An overview.Journal of biosciences · 2020Article
- In-planta expression of insecticidal proteins provides protection against lepidopteran insects.Scientific reports · 2019Article
- Expression of hybrid fusion protein (Cry1Ac::ASAL) in transgenic rice plants imparts resistance against multiple insect pests.Scientific reports · 2018Article
- Strength, Stability, and cis-Motifs of In silico Identified Phloem-Specific Promoters in Brassica juncea (L.).Frontiers in plant science · 2016Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Recombinant fusion proteins containing arthropod toxins have been developed as a new class of biopesticides. The recombinant fusion protein Hv1a/GNA, containing the spider venom toxin ω-ACTX-Hv1a linked to snowdrop lectin (GNA) was shown to reduce survival of the peach-potato aphid Myzus persicae when delivered in artificial diet, with survival <10% after 8 days exposure to fusion protein at 1 mg/ml. Although the fusion protein was rapidly degraded by proteases in the insect, Hv1a/GNA oral toxicity to M. persicae was significantly greater than GNA alone. A construct encoding the fusion protein, including the GNA leader sequence, under control of the constitutive CaMV 35S promoter was transformed into Arabidopsis; the resulting plants contained intact fusion protein in leaf tissues at an estimated level of 25.6 ± 4.1 ng/mg FW. Transgenic Arabidopsis expressing Hv1a/GNA induced up to 40% mortality of M. persicae after 7 days exposure in detached leaf bioassays, demonstrating that transgenic plants can deliver fusion proteins to aphids. Grain aphids (Sitobion avenae) were more susceptible than M. persicae to the Hv1a/GNA fusion protein in artificial diet bioassays (LC50 = 0.73 mg/ml after 2 days against LC50 = 1.81 mg/ml for M. persicae), as they were not able to hydrolyze the fusion protein as readily as M. persicae. Expression of this fusion protein in suitable host plants for the grain aphid is likely to confer higher levels of resistance than that shown with the M. persicae/Arabidopsis model system.
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