ArticleBiotechnology for biofuels2014
Engineering of an endogenous hexose transporter into a specific D-xylose transporter facilitates glucose-xylose co-consumption in Saccharomyces cerevisiae.
Article in Biotechnology for biofuels, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 125 citations in OpenAlex.
- Engineering the acid-tolerant yeastFrontiers in microbiology · 2026Article
- Recent progress in engineering yeast producers of cellulosic ethanol.FEMS yeast research · 2025Review
- Molecular Modification Enhances Xylose Uptake by the Sugar Transporter KM_SUT5 ofInternational journal of molecular sciences · 2024Article
- Compositional and temporal division of labor modulates mixed sugar fermentation by an engineered yeast consortium.Nature communications · 2024Article
- Engineering of Ogataea polymorpha strains with ability for high-temperature alcoholic fermentation of cellobiose.FEMS yeast research · 2024Article
- Maltose accumulation-induced cell death in Saccharomyces cerevisiae.FEMS yeast research · 2024Article
- Structural and biochemical insights of xylose MFS and SWEET transporters in microbial cell factories: challenges to lignocellulosic hydrolysates fermentation.Frontiers in microbiology · 2024Review
- Metabolic Engineering ofJournal of fungi (Basel, Switzerland) · 2023Article
- D-xylose accelerated death of pentose metabolizing Saccharomyces cerevisiae.Biotechnology for biofuels and bioproducts · 2023Article
- Review
- Using phosphoglucose isomerase-deficient (pgi1Δ) Saccharomyces cerevisiae to map the impact of sugar phosphate levels on D-glucose and D-xylose sensing.Microbial cell factories · 2022Article
- 2-deoxyglucose transiently inhibits yeast AMPK signaling and triggers glucose transporter endocytosis, potentiating the drug toxicity.PLoS genetics · 2022Article
- Combined roles of exporters in acetic acid tolerance in Saccharomyces cerevisiae.Biotechnology for biofuels and bioproducts · 2022Article
- Machine learning and comparative genomics approaches for the discovery of xylose transporters in yeast.Biotechnology for biofuels and bioproducts · 2022Article
- Novel Evolutionary Engineering Approach to Alter Substrate Specificity of Disaccharide Transporter Mal11 inJournal of fungi (Basel, Switzerland) · 2022Article
- Physiological limitations and opportunities in microbial metabolic engineering.Nature reviews. Microbiology · 2022Review
- Identification of a glucose-insensitive variant of Gal2 from Saccharomyces cerevisiae exhibiting a high pentose transport capacity.Scientific reports · 2021Article
- Valorisation of xylose to renewable fuels and chemicals, an essential step in augmenting the commercial viability of lignocellulosic biorefineries.Sustainable energy & fuels · 2021Review
- D-Xylose Sensing inInternational journal of molecular sciences · 2021Review
- D-glucose overflow metabolism in an evolutionary engineered high-performance D-xylose consuming Saccharomyces cerevisiae strain.FEMS yeast research · 2021Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEngineering of Saccharomyces cerevisiae for the simultaneous utilization of hexose and pentose sugars is vital for cost-efficient cellulosic bioethanol production. This yeast lacks specific pentose transporters and depends on endogenous hexose transporters for low affinity pentose uptake. Consequently, engineered xylose-fermenting yeast strains first utilize D-glucose before D-xylose can be transported and metabolized.
resultsWe have used an evolutionary engineering approach that depends on a quadruple hexokinase deletion xylose-fermenting S. cerevisiae strain to select for growth on D-xylose in the presence of high D-glucose concentrations. This resulted in D-glucose-tolerant growth of the yeast of D-xylose. This could be attributed to mutations at N367 in the endogenous chimeric Hxt36 transporter, causing a defect in D-glucose transport while still allowing specific uptake of D-xylose. The Hxt36-N367A variant transports D-xylose with a high rate and improved affinity, enabling the efficient co-consumption of D-glucose and D-xylose.
conclusionsEngineering of yeast endogenous hexose transporters provides an effective strategy to construct glucose-insensitive xylose transporters that are well integrated in the carbon metabolism regulatory network, and that can be used for efficient lignocellulosic bioethanol production.
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