Evidence map›Paper›PMID 25505699›Full record

ArticleTranslational respiratory medicine2014

Inhibition of β-catenin/p300 interaction proximalizes mouse embryonic lung epithelium.

Tomoyo Sasaki, Michael Kahn

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Translational respiratory medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
1.2field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 22 citations in OpenAlex.

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  13. WNT Signaling in Cardiac and Vascular Disease.Pharmacological reviews · 2018
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Tomoyo SasakiDepartment of Biochemistry and Molecular Biology, Keck School of Medicine, University of Southern California, 1450 Biggy Street, Los Angeles, CA 90033 USA.
Michael KahnDepartment of Biochemistry and Molecular Biology, Keck School of Medicine, University of Southern California, 1450 Biggy Street, Los Angeles, CA 90033 USA ; Center for Molecular Pathways and Drug Discovery, Keck School of Medicine, University of Southern California, 1450 Biggy Street, Los Angeles, CA 90033 USA ; Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, 1450 Biggy Street, Los Angeles, CA 90033 USA.
University of Southern California · US

Funding

Efficacy of Novel Wnt Modulator in Colon CancerR01CA166161 · NCI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI KAHN, MICHAEL, LENZ, HEINZ JOSEF · 2013 to 2017
$2.3M
Mechanisms of beta-catenin signaling in alveolar epithelial cell differentiationR01HL112638 · NHLBI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI BOROK, ZEA, KAHN, MICHAEL · 2013 to 2016
$2.2M
NCI NIH HHS R01 CA166161NHLBI NIH HHS R01 HL112638
6 · The paper itself

Abstract

backgroundWnt/β-catenin signaling has been suggested to regulate proximal-distal determination of embryonic lung epithelium based upon genetically modified mouse models. The previously identified and characterized small molecule inhibitor IQ1 can pharmacologically decrease the interaction between β-catenin and its transcriptional coactivator p300, thereby enhancing the β-catenin/CBP interaction. Inhibition of the β-catenin/p300 interaction by IQ1 blocks the differentiation of embryonic stem cells and epicardial progenitor cells; however, whether differential coactivator usage by β-catenin plays a role in proximal-distal determination of lung epithelium is unknown.

methodsWe examined the effects of inhibiting the β-catenin/p300 interaction with IQ1 on lung branching morphogenesis in mouse embryos in utero and mouse embryonic lung organ culture ex vivo. The phenotype of IQ1 treated lungs was analyzed by epithelial staining, histology, quantitative PCR and in situ hybridization.

resultsInhibition of the β-catenin/p300 interaction by IQ1 disrupted the distal branching of mouse lung epithelium both in utero and ex vivo. IQ1 proximalized lung epithelium with decreased expression of the genes Bmp4 and Fgf10, hallmarks of distal lung determination, and increased expression of the proximal genes Sox2 and Scgb1a1 (CC10) as shown by quantitative PCR and in situ hybridization. The disruption of branching was reversible ex vivo as branching was reinitiated after removal of IQ1 from the media.

conclusionsThe results demonstrate that the β-catenin/p300 interaction plays a critical role in proximal-distal determination of the epithelium in mouse lung branching morphogenesis and β-catenin/p300 inhibition pharmacologically proximalizes lung epithelium.

Indexed as

BranchingCBPDifferentiationICG-001IQ1Lungp300Small molecule inhibitorWntβ-catenin

Identifiers

PMID25505699
PMCPMC4229507
OpenAlexW2157406619

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.