ArticleFrontiers in genetics2014
Validation of a multiplex reverse transcription and pre-amplification method using TaqMan(®) MicroRNA assays.
Article in Frontiers in genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT04703647 (Longitudinal Follow-up Study About Complex Regional Pain Syndrome), which is not on this map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Longitudinal Follow-up Study About Complex Regional Pain Syndrome (CRPS). Which Are the Predictors for Return to Work and Recovery
Who cites it
24 citing papers in PubMed.
- miR-19b-3p is associated with a diametric response to resistance exercise in older adults and regulates skeletal muscle anabolism via PTEN inhibition.American journal of physiology. Cell physiology · 2021Trial
- An Optimized RT-qPCR Protocol for Comprehensive Analysis of microRNAs and mRNAs inNon-coding RNA · 2026Article
- Analysis of MicroRNAs Expression Across Cells, Tissues, and Body Fluids.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Evaluating the expression level of serum Interleukin-2, lipoarabinomannan and circulating MicroRNA-29a as diagnostic biomarkers for pulmonary and extra-pulmonary tuberculosis: a pilot study.Annals of medicine · 2025Article
- The Role of miRNAs and Extracellular Vesicles in Adaptation After Resistance Exercise: A Review.Current issues in molecular biology · 2025Review
- MicroRNA-452-5p regulates fibrogenesis via targeting TGF-β/SMAD4 axis in SCN5A-knockdown human cardiac fibroblasts.iScience · 2024Article
- Archival skin biopsy specimens as a tool for miRNA-based diagnosis: Technical and post-analytical considerations.Molecular therapy. Methods & clinical development · 2023Article
- Protein tyrosine phosphatase 1B regulates miR-208b-argonaute 2 association and thyroid hormone responsiveness in cardiac hypertrophy.Science signaling · 2022Article
- Triple-Helical Binding of Peptide Nucleic Acid Inhibits Maturation of Endogenous MicroRNA-197.ACS chemical biology · 2021Article
- Diagnostic potential of three serum microRNAs as biomarkers for equine sarcoid disease in horses and donkeys.Journal of veterinary internal medicine · 2021Article
- Extracellular vesicular miRNA expression is not a proxy for skeletal muscle miRNA expression in males and females following acute, moderate intensity exercise.Physiological reports · 2020Article
- T cell exosome-derived miR-142-3p impairs glandular cell function in Sjögren's syndrome.JCI insight · 2020Article
- High-intensity interval exercise increases humanin, a mitochondrial encoded peptide, in the plasma and muscle of men.Journal of applied physiology (Bethesda, Md. : 1985) · 2020Article
- Article
- Analysis of miRNA signatures in CSF identifies upregulation of miR-21 and miR-146a/b in patients with multiple sclerosis and active lesions.Journal of neuroinflammation · 2019Article
- West Nile virus infection and interferon alpha treatment alter the spectrum and the levels of coding and noncoding host RNAs secreted in extracellular vesicles.BMC genomics · 2019Article
- Downregulation of intratumoral expression of miR-205, miR-200c and miR-125b in primary human cutaneous melanomas predicts shorter survival.Scientific reports · 2018Article
- MicroRNA.The Journal of allergy and clinical immunology · 2018Review
- Article
- Modified Polyadenylation-Based RT-qPCR Increases Selectivity of Amplification of 3'-MicroRNA Isoforms.Frontiers in genetics · 2018Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Since the discovery of microRNAs (miRNAs), different approaches have been developed to label, amplify and quantify miRNAs. The TaqMan(®) technology, provided by Applied Biosystems (ABIs), uses a stem-loop reverse transcription primer system to reverse transcribe the RNA and amplify the cDNA. This method is widely used to identify global differences between the expression of 100s of miRNAs across comparative samples. This technique also allows the quantification of the expression of targeted miRNAs to validate observations determined by whole-genome screening or to analyze few specific miRNAs on a large number of samples. Here, we describe the validation of a method published by ABIs on their web site allowing to reverse transcribe and pre-amplify multiple miRNAs and snoRNAs simultaneously. The validation of this protocol was performed on human muscle and plasma samples. Fast and cost efficient, this method achieves an easy and convenient way to screen a relatively large number of miRNAs in parallel.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.