ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2014
Live imaging of endogenous PSD-95 using ENABLED: a conditional strategy to fluorescently label endogenous proteins.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed.
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- Scales of Postsynaptic Nanostructure: Molecules, Nanoclusters, and Nanodomains.Advances in neurobiology · 2026Review
- Single-cell endogenous protein labeling via CRISPR-Cas9-mediated genome editing in the mouse brain.Anatomical science international · 2025Article
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- Early hippocampal hyperexcitability and synaptic reorganization in mouse models of amyloidosis.iScience · 2024Article
- Synaptic input and CaNature neuroscience · 2024Article
- Amyloid Pathology Impairs Experience-Dependent Inhibitory Synaptic Plasticity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
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- Single-cell synaptome mapping: its technical basis and applications in critical period plasticity research.Frontiers in neural circuits · 2024Review
- Antiretroviral drugs from multiple classes induce loss of excitatory synapses between hippocampal neurons in culture.Frontiers in pharmacology · 2024Article
- Recent advances and challenges in the use of CRISPR/Cas9 genome editing for understanding neuronal cell biology.Neurophotonics · 2023Article
- Amyloid pathology impairs experience-dependent inhibitory synaptic plasticity.bioRxiv : the preprint server for biology · 2023Article
- Btbd11 supports cell-type-specific synaptic function.Cell reports · 2023Article
- Simple and Highly Efficient Detection of PSD95 Using a Nanobody and Its Recombinant Heavy-Chain Antibody Derivatives.International journal of molecular sciences · 2023Article
- Quantitative Fluorescence Analysis Reveals Dendrite-Specific Thalamocortical Plasticity in L5 Pyramidal Neurons during Learning.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
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12 authors.
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Abstract
Stoichiometric labeling of endogenous synaptic proteins for high-contrast live-cell imaging in brain tissue remains challenging. Here, we describe a conditional mouse genetic strategy termed endogenous labeling via exon duplication (ENABLED), which can be used to fluorescently label endogenous proteins with near ideal properties in all neurons, a sparse subset of neurons, or specific neuronal subtypes. We used this method to label the postsynaptic density protein PSD-95 with mVenus without overexpression side effects. We demonstrated that mVenus-tagged PSD-95 is functionally equivalent to wild-type PSD-95 and that PSD-95 is present in nearly all dendritic spines in CA1 neurons. Within spines, while PSD-95 exhibited low mobility under basal conditions, its levels could be regulated by chronic changes in neuronal activity. Notably, labeled PSD-95 also allowed us to visualize and unambiguously examine otherwise-unidentifiable excitatory shaft synapses in aspiny neurons, such as parvalbumin-positive interneurons and dopaminergic neurons. Our results demonstrate that the ENABLED strategy provides a valuable new approach to study the dynamics of endogenous synaptic proteins in vivo.
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