ArticleMolecular & cellular proteomics : MCP2015
A chemical proteomics approach for global analysis of lysine monomethylome profiling.
Article in Molecular & cellular proteomics : MCP, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 76 citations in OpenAlex.
- Article
- Utilizing a Negative Enrichment Strategy to Profile Protein Methylation, Leveraging the Orthogonality of LysargiNase and Trypsin.Molecular & cellular proteomics : MCP · 2025Article
- Quantitative Analysis of Nonhistone Lysine Methylation Sites and Lysine Demethylases in Breast Cancer Cell Lines.Journal of proteome research · 2025Article
- Decoding the protein methylome: Identification, validation, and functional insights.Bioorganic & medicinal chemistry · 2025Review
- Quantitative analysis of non-histone lysine methylation sites and lysine demethylases in breast cancer cell lines.bioRxiv : the preprint server for biology · 2024Article
- Protein Thermal Stability Changes Induced by the Global Methylation Inhibitor 3-Deazaneplanocin A (DZNep).Biomolecules · 2024Article
- Substrate and Functional Diversity of Protein Lysine Post-translational Modifications.Genomics, proteomics & bioinformatics · 2024Review
- Protein methylation in mitochondria.The Journal of biological chemistry · 2022Review
- Histone H3 N-terminal mimicry drives a novel network of methyl-effector interactions.The Biochemical journal · 2021Article
- Identification of lysine isobutyrylation as a new histone modification mark.Nucleic acids research · 2021Article
- Characterization of Lysine Monomethylome and Methyltransferase in Model Cyanobacterium Synechocystis sp. PCC 6803.Genomics, proteomics & bioinformatics · 2020Article
- MeCP2 and Chromatin Compartmentalization.Cells · 2020Review
- Quantitative analysis of protein crotonylation identifies its association with immunoglobulin A nephropathy.Molecular medicine reports · 2020Article
- Deep Protein Methylation Profiling by Combined Chemical and Immunoaffinity Approaches Reveals Novel PRMT1 Targets.Molecular & cellular proteomics : MCP · 2019Article
- Lysine Methylation Regulators Moonlighting outside the Epigenome.Molecular cell · 2019Review
- Sample Multiplexing Strategies in Quantitative Proteomics.Analytical chemistry · 2019Review
- Quantitative analysis of global protein lysine methylation by mass spectrometry.Methods in enzymology · 2019Article
- A qualitative proteome-wide lysine crotonylation profiling of papaya (Carica papaya L.).Scientific reports · 2018Article
- METTL21B Is a Novel Human Lysine Methyltransferase of Translation Elongation Factor 1A: Discovery by CRISPR/Cas9 Knockout.Molecular & cellular proteomics : MCP · 2017Article
- The first succinylome profile of Trichophyton rubrum reveals lysine succinylation on proteins involved in various key cellular processes.BMC genomics · 2017Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Methylation of lysine residues on histone proteins is known to play an important role in chromatin structure and function. However, non-histone protein substrates of this modification remain largely unknown. An effective approach for system-wide analysis of protein lysine methylation, particularly lysine monomethylation, is lacking. Here we describe a chemical proteomics approach for global screening for monomethyllysine substrates, involving chemical propionylation of monomethylated lysine, affinity enrichment of the modified monomethylated peptides, and HPLC/MS/MS analysis. Using this approach, we identified with high confidence 446 lysine monomethylation sites in 398 proteins, including three previously unknown histone monomethylation marks, representing the largest data set of protein lysine monomethylation described to date. Our data not only confirms previously discovered lysine methylation substrates in the nucleus and spliceosome, but also reveals new substrates associated with diverse biological processes. This method hence offers a powerful approach for dynamic study of protein lysine monomethylation under diverse cellular conditions and in human diseases.
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