Evidence map›Paper›PMID 25505007›Full record

ArticlePlant & cell physiology2015

MOROKOSHI: transcriptome database in Sorghum bicolor.

Yuko Makita, Setsuko Shimada, Mika Kawashima, Tomoko Kondou-Kuriyama, Tetsuro Toyoda, Minami Matsui

Open access · bronzeAbstract read
In one paragraph

Article in Plant & cell physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.

0numbers the graph read from it
0cells of the map it votes in
74citing papers in PubMed
4.3field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

74 citing papers in PubMed, 141 citations in OpenAlex.

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14 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Yuko MakitaSynthetic Genomics Research Team, Biomass Research Cooperation Division (BMEP), RIKEN Center for Sustainable Resource Science (CSRS), 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045 Japan.
Setsuko ShimadaSynthetic Genomics Research Team, Biomass Research Cooperation Division (BMEP), RIKEN Center for Sustainable Resource Science (CSRS), 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045 Japan.
Mika KawashimaSynthetic Genomics Research Team, Biomass Research Cooperation Division (BMEP), RIKEN Center for Sustainable Resource Science (CSRS), 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045 Japan.
Tomoko Kondou-KuriyamaSynthetic Genomics Research Team, Biomass Research Cooperation Division (BMEP), RIKEN Center for Sustainable Resource Science (CSRS), 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045 Japan.
Tetsuro ToyodaRIKEN Advanced Center for Computing and Communication (ACCC), Hirosawa 2-1, Wako, Saitama, 351-0198 Japan.
Minami MatsuiSynthetic Genomics Research Team, Biomass Research Cooperation Division (BMEP), RIKEN Center for Sustainable Resource Science (CSRS), 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045 Japan minami@riken.jp.
RIKEN Center for Sustainable Resource Science · JPRIKEN Center for Advanced Photonics · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In transcriptome analysis, accurate annotation of each transcriptional unit and its expression profile is essential. A full-length cDNA (FL-cDNA) collection facilitates the refinement of transcriptional annotation, and accurate transcription start sites help to unravel transcriptional regulation. We constructed a normalized FL-cDNA library from eight growth stages of aerial tissues in Sorghum bicolor and isolated 37,607 clones. These clones were Sanger sequenced from the 5' and/or 3' ends and in total 38,981 high-quality expressed sequence tags (ESTs) were obtained. About one-third of the transcripts of known genes were captured as FL-cDNA clone resources. In addition to these, we also annotated 272 novel genes, 323 antisense transcripts and 1,672 candidate isoforms. These clones are available from the RIKEN Bioresource Center. After obtaining accurate annotation of transcriptional units, we performed expression profile analysis. We carried out spikelet-, seed- and stem-specific RNA sequencing (RNA-Seq) analysis and confirmed the expression of 70.6% of the newly identified genes. We also downloaded 23 sorghum RNA-Seq samples that are publicly available and these are shown on a genome browser together with our original FL-cDNA and RNA-Seq data. Using our original and publicly available data, we made an expression profile of each gene and identified the top 20 genes with the most similar expression. In addition, we visualized their relationships in gene co-expression networks. Users can access and compare various transcriptome data from S, bicolor at http://sorghum.riken.jp.

Indexed as

Gene Expression Regulation, PlantTranscriptomeBase SequenceDNA, ComplementaryExpressed Sequence TagsFlowersGene ExpressionGene Expression ProfilingGene LibraryGenome, PlantOrgan SpecificityPlant StemsRNA, MessengerRNA, PlantSeedsSequence Analysis, RNADNA, ComplementaryRNA, MessengerRNA, PlantDatabaseFL-cDNANew transcriptNGSPlantSorghum

Identifiers

PMID25505007
PMCPMC4301747
OpenAlexW1995120919

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.