ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2015
Generation and characterization of tribenuron-methyl herbicide-resistant rapeseed (Brasscia napus) for hybrid seed production using chemically induced male sterility.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 43 citations in OpenAlex.
- Genetic Origin ofPlants (Basel, Switzerland) · 2026Article
- A chaperonin BnaC01.CCT8 contributes to silique length and seed weight by affecting auxin and jasmonic acid signalling in Brassica napus.Plant biotechnology journal · 2025Article
- Molecular Mechanisms of Herbicide Resistance in Rapeseed: Current Status and Future Prospects for Resistant Germplasm Development.International journal of molecular sciences · 2025Review
- Identification and Characterization of a Male Sterile Rapeseed (Plants (Basel, Switzerland) · 2025Article
- Genetic characterization of the AHAS mutant line K4 with resistance to AHAS-inhibitor herbicides in rapeseed (Brassica napus L.).Stress biology · 2025Article
- Comparative genome-wide analysis of circular RNAs in Brassica napus L.: target-site versus non-target-site resistance to herbicide stress.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- A CRISPR/Cas9-induced male-sterile line facilitating easy hybrid production in polyploid rapeseed (Horticulture research · 2024Article
- The story of a decade: Genomics, functional genomics, and molecular breeding in Brassica napus.Plant communications · 2024Review
- BnaC01.BIN2, a GSK3-like kinase, modulates plant height and yield potential in Brassica napus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- A LuALS Mutation with High Sulfonylurea Herbicide Resistance inInternational journal of molecular sciences · 2023Article
- Development and validation of functional kompetitive allele-specific PCR markers for herbicide resistance inFrontiers in plant science · 2023Article
- Unraveling the Genetic Basis of Fertility Restoration for Cytoplasmic Male Sterile LineFrontiers in plant science · 2021Article
- Synergistic mutations of two rapeseed AHAS genes confer high resistance to sulfonylurea herbicides for weed control.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- Inheritance and Molecular Characterization of a Novel MutatedInternational journal of molecular sciences · 2020Article
- Sublethal application of various sulfonylurea and imidazolinone herbicides favors outcrossing and hybrid seed production in oilseed rape.BMC plant biology · 2020Article
- Precision Genome Engineering Through Cytidine Base Editing in Rapeseed (Frontiers in genome editing · 2020Article
- Fine-mapping and transcriptome analysis of a candidate gene controlling plant height inBiotechnology for biofuels · 2020Article
- Overcoming Cabbage Crossing Incompatibility by the Development and Application of Self-Compatibility-QTL- Specific Markers and Genome-Wide Background Analysis.Frontiers in plant science · 2019Article
- The initial deficiency of protein processing and flavonoids biosynthesis were the main mechanisms for the male sterility induced by SX-1 in Brassica napus.BMC genomics · 2018Article
- Male Sterility of anFrontiers in plant science · 2018Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageIdentification and molecular analysis of four tribenuron-methyl resistant mutants in Brassica napus , which would be very useful in hybrid production using a Chemically induced male sterility system. Chemically induced male sterility (CIMS) systems dependent on chemical hybridization agents (CHAs) like tribenuron-methyl (TBM) represent an important approach for practical utilization of heterosis in rapeseed. However, when spraying the female parents with TBM to induce male sterility the male parents must be protected with a shield to avoid injury to the stamens, which would otherwise complicate the seed production protocol and increase the cost of hybrid seed production. Here we report the first proposed application of a herbicide-resistant cultivar in hybrid production, using a CIMS system based on identifying four TBM-resistant mutants in Brassica napus. Genetic analysis indicated that the TBM resistance was controlled by a single dominant nuclear gene. An in vitro enzyme activity assay for acetohydroxyacid synthase (AHAS) suggested that the herbicide resistance is caused by a gain-of-function mutation in a copy of AHAS genes. Comparative sequencing of the mutants and wild type BnaA.AHAS.a coding sequences identified a C-to-T transition at either position 535 or 536 from the translation start site, which resulted in a substitution of proline with serine or leucine at position 197 according to the Arabidopsis thaliana protein sequence. An allele-specific dCAPS marker developed from the C536T variation co-segregated with the herbicide resistance. Transgenic A. thaliana plants expressing BnaA.ahas3.a conferred herbicide resistance, which confirmed that the P197 substitution in BnaA.AHAS.a was responsible for the herbicide resistance. Moreover, the TBM-resistant lines maintain normal male fertility under TBM treatment and can be of practical value in hybrid seed production using CIMS.
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