ArticleNature protocols2015
Genome-scale RNAi screens for high-throughput phenotyping in bloodstream-form African trypanosomes.
Article in Nature protocols, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
- Zinc-dependent turnover of ZIP3 transporter mRNA by trypanosome ZNK1.Nucleic acids research · 2026Article
- A genome-wide genetic screen identifies a novel kDNA replication protein in trypanosomes.Nucleic acids research · 2026Article
- A non-coding role for trypanosome VSG transcripts in allelic exclusion.Nucleic acids research · 2025Article
- Article
- A lineage-specific protein network at the trypanosome nuclear envelope.Nucleus (Austin, Tex.) · 2024Article
- Post-transcriptional reprogramming by thousands of mRNA untranslated regions in trypanosomes.Nature communications · 2024Article
- Phenotypic screening reveals a highly selective phthalimide-based compound with antileishmanial activity.PLoS neglected tropical diseases · 2024Article
- Genome-wide screens connect HD82 loss-of-function to purine analog resistance in African trypanosomes.mSphere · 2024Article
- Deep mutational scanning of the RNase III-like domain inFrontiers in cellular and infection microbiology · 2024Article
- An allele-selective inter-chromosomal protein bridge supports monogenic antigen expression in the African trypanosome.Nature communications · 2023Article
- Development of a 2,4-Diaminothiazole Series for the Treatment of Human African Trypanosomiasis Highlights the Importance of Static-Cidal Screening of Analogues.Journal of medicinal chemistry · 2023Article
- Genome-scale RNA interference profiling of Trypanosoma brucei cell cycle progression defects.Nature communications · 2022Article
- Article
- Repurposing Lansoprazole and Posaconazole to treat leishmaniasis: Integration of in vitro testing, pharmacological corroboration, and mechanisms of action.Journal of food and drug analysis · 2022Article
- Multiple unbiased approaches identify oxidosqualene cyclase as the molecular target of a promising anti-leishmanial.Cell chemical biology · 2021Article
- 4E Interacting Protein as a Potential Novel Drug Target for Nucleoside Analogues inMicroorganisms · 2021Article
- TbSAP is a novel chromatin protein repressing metacyclic variant surface glycoprotein expression sites in bloodstream form Trypanosoma brucei.Nucleic acids research · 2021Article
- Spatial integration of transcription and splicing in a dedicated compartment sustains monogenic antigen expression in African trypanosomes.Nature microbiology · 2021Article
- Reliable, scalable functional genetics in bloodstream-form Trypanosoma congolense in vitro and in vivo.PLoS pathogens · 2021Article
- Veterinary trypanocidal benzoxaboroles are peptidase-activated prodrugs.PLoS pathogens · 2020Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
The ability to simultaneously assess every gene in a genome for a role in a particular process has obvious appeal. This protocol describes how to perform genome-scale RNAi library screens in bloodstream-form African trypanosomes, a family of parasites that causes lethal human and animal diseases and also serves as a model for studies on basic aspects of eukaryotic biology and evolution. We discuss strain assembly, screen design and implementation, the RNAi target sequencing approach and hit validation, and we provide a step-by-step protocol. A screen can yield from one to thousands of 'hits' associated with the phenotype of interest. The screening protocol itself takes 2 weeks or less to be completed, and high-throughput sequencing may also be completed within weeks. Pre- and post-screen strain assembly, validation and follow-up can take several months, depending on the type of screen and the number of hits analyzed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.