Evidence map›Paper›PMID 25502886›Full record

ArticleNature protocols2015

Particle generation, functionalization and sortase A-mediated modification with targeting of single-chain antibodies for diagnostic and therapeutic use.

Christoph E Hagemeyer, Karen Alt, Angus P R Johnston, Georgina K Such, Hang T Ta, Melissa K M Leung, Sandeep Prabhu, Xiaowei Wang, Frank Caruso, Karlheinz Peter

Abstract read
PubMed Publisher
In one paragraph

Article in Nature protocols, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Article
  2. Nanoparticles for vaccine and gene therapy: Overcoming the barriers to nucleic acid delivery.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2022
    Review
  3. Review
  4. Review
  5. Review
  6. Review
  7. Dual-Targeted Theranostic Delivery of miRs Arrests Abdominal Aortic Aneurysm Development.Molecular therapy : the journal of the American Society of Gene Therapy · 2018
    Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Christoph E Hagemeyer1] Vascular Biotechnology Laboratory, Baker IDI Heart and Diabetes Institute, Melbourne, Victoria, Australia. [2] Central Clinical School, Monash University, Melbourne, Victoria, Australia.
Karen Alt1] Vascular Biotechnology Laboratory, Baker IDI Heart and Diabetes Institute, Melbourne, Victoria, Australia. [2] Atherothrombosis and Vascular Biology Laboratory, Baker IDI Heart and Diabetes Institute, Melbourne, Victoria, Australia.
Angus P R JohnstonDepartment of Drug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University, Melbourne, Victoria, Australia.ORCID 0000000156114515
Georgina K SuchDepartment of Chemistry, The University of Melbourne, Melbourne, Victoria, Australia.
Hang T Ta1] Atherothrombosis and Vascular Biology Laboratory, Baker IDI Heart and Diabetes Institute, Melbourne, Victoria, Australia. [2] Australian Institute of Bioengineering and Nanotechnology, The University of Queensland, Brisbane, Queensland, Australia.
Melissa K M LeungDepartment of Chemical and Biomolecular Engineering, The University of Melbourne, Melbourne, Victoria, Australia.
Sandeep Prabhu1] Central Clinical School, Monash University, Melbourne, Victoria, Australia. [2] Atherothrombosis and Vascular Biology Laboratory, Baker IDI Heart and Diabetes Institute, Melbourne, Victoria, Australia.
Xiaowei Wang1] Central Clinical School, Monash University, Melbourne, Victoria, Australia. [2] Atherothrombosis and Vascular Biology Laboratory, Baker IDI Heart and Diabetes Institute, Melbourne, Victoria, Australia.
Frank CarusoDepartment of Chemical and Biomolecular Engineering, The University of Melbourne, Melbourne, Victoria, Australia.
Karlheinz Peter1] Central Clinical School, Monash University, Melbourne, Victoria, Australia. [2] Atherothrombosis and Vascular Biology Laboratory, Baker IDI Heart and Diabetes Institute, Melbourne, Victoria, Australia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Antibody fusion to nonprotein materials such as contrast agents or radio-tracers, nano- or microparticles or small-molecule drugs is attracting major interest for molecular imaging and drug delivery. Nondirected bioconjugation techniques may impair antibody affinity, result in lower amounts of functional antibodies and generate multicomponent mixtures. We present a detailed protocol for the enzymatic bioconjugation of small recombinant antibodies to imaging particles, and we also describe the generation of and conjugation to a low-fouling capsule assembled for drug delivery from PEG and PVPON (poly(N-vinylpyrrolidone) by a layer-by-layer (LbL) technique. The single-chain variable fragment (scFv) is equipped with a short C-terminal LPETG tag and the fusion partners are functionalized with an N-terminal GGG nucleophilic group for sortase A conjugation. The LbL capsules are assembled through hydrogen bonding by depositing alkyne-modified poly(vinylpyrrolidone) and poly(methacrylic acid) layers on silica particles, followed by depositing alkyne-modified PEG. The generation of the antibodies and LbL capsules takes ∼1-2 weeks each. The conjugation and functional testing takes another 3-4 d.

Indexed as

AminoacyltransferasesBacterial ProteinsCysteine EndopeptidasesDrug Delivery SystemsHydrogen BondingMolecular ImagingMolecular StructureNanoparticlesPolyethylene GlycolsPolymethacrylic AcidsPolyvinylsPyrrolidinesSingle-Chain AntibodiesAminoacyltransferasesBacterial ProteinsCysteine EndopeptidasesPolyethylene GlycolsPolymethacrylic Acidspoly(N-vinylpyrrolidine)PolyvinylsPyrrolidinesSingle-Chain Antibodiessortase A

Identifiers

PMID25502886

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.