ArticleNature protocols2015
Quantitative assessment of angiogenesis, perfused blood vessels and endothelial tip cells in the postnatal mouse brain.
Article in Nature protocols, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
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Who cites it
79 citing papers in PubMed, 127 citations in OpenAlex.
- Adenylosuccinate acts as a novel pro-angiogenic factor.Research square · 2026Article
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- PAVSAT: an automated blood vessel analysis tool using deep learning-based segmentation and image processing.BMC bioinformatics · 2026Article
- Pericytes are organ-specific regulators of tissue morphogenesis.Nature communications · 2026Article
- Neuronal MCT2 promotes angiogenesis via lactate in the developing mouse neocortex.Cell death and differentiation · 2026Article
- DNA replication fork speed acts as a pacer in cortical neurogenesis.Nature communications · 2025Article
- Edge participation coefficient unveiling the developmental dynamics of neonatal functional connectome.Communications biology · 2025Article
- Advanced hydrogel mesh platform with neural stem cells and human umbilical vein endothelial cells for enhanced axonal regeneration.APL bioengineering · 2025Article
- Blood Progenitor Cell Mobilization Driven by TWEAK Promotes Neovascularization and Reduces Brain Damage in a Rat Model of Intracerebral Hemorrhage.Antioxidants (Basel, Switzerland) · 2025Article
- CardiLect: A combined cross-species lectin histochemistry protocol for the automated analysis of cardiac remodelling.ESC heart failure · 2025Article
- Species-specific blood-brain barrier permeability in amphibians.BMC biology · 2025Article
- Drugless peptide-based nanohybrids alleviate diabetic retinopathy by suppressing microglial activation and endothelial inflammation.Theranostics · 2025Article
- CRISPR/CasRx suppresses KRAS-induced brain arteriovenous malformation developed in postnatal brain endothelial cells in mice.JCI insight · 2024Article
- Protein O-GlcNAcylation coupled to Hippo signaling drives vascular dysfunction in diabetic retinopathy.Nature communications · 2024Article
- Crucial role of Aquaporin-4 extended isoform in brain water Homeostasis and Amyloid-β clearance: implications for Edema and neurodegenerative diseases.Acta neuropathologica communications · 2024Article
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- Neurovascular and immune factors of vulnerability of substantia nigra dopaminergic neurons in non-human primates.NPJ Parkinson's disease · 2024Article
- The liver and muscle secreted HFE2-protein maintains central nervous system blood vessel integrity.Nature communications · 2024Article
- Reduced Brain Cortex Angiogenesis in the Offspring of the Preeclampsia-Like Syndrome.Hypertension (Dallas, Tex. : 1979) · 2023Article
- VEGFR2 Expression Correlates with Postnatal Development of Brain Arteriovenous Malformations in a Mouse Model of Type I Hereditary Hemorrhagic Telangiectasia.Biomedicines · 2023Article
19 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During development and in various diseases of the CNS, new blood vessel formation starts with endothelial tip cell selection and vascular sprout migration, followed by the establishment of functional, perfused blood vessels. Here we describe a method that allows the assessment of these distinct angiogenic steps together with antibody-based protein detection in the postnatal mouse brain. Intravascular and perivascular markers such as Evans blue (EB), isolectin B4 (IB4) or laminin (LN) are used alongside simultaneous immunofluorescence on the same sections. By using confocal laser-scanning microscopy and stereological methods for analysis, detailed quantification of the 3D postnatal brain vasculature for perfused and nonperfused vessels (e.g., vascular volume fraction, vessel length and number, number of branch points and perfusion status of the newly formed vessels) and characterization of sprouting activity (e.g., endothelial tip cell density, filopodia number) can be obtained. The entire protocol, from mouse perfusion to vessel analysis, takes ∼10 d.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.