ArticlePlastic and reconstructive surgery2015
Studies in fat grafting: Part IV. Adipose-derived stromal cell gene expression in cell-assisted lipotransfer.
Article in Plastic and reconstructive surgery, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
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Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it, 76 citations in OpenAlex.
- Cell-Assisted Lipotransfer: A Systematic Review of Its Efficacy.Aesthetic plastic surgery · 2016Pooled it
- Autologous adipose tissue transfer in progressive hemifacial atrophy: From simple volume to regenerative cell therapy.JPRAS open · 2025Review
- Fat Grafting With Lymphedema Fat: From Trash to Treasure?Plastic and reconstructive surgery. Global open · 2025Article
- A Protocol for co-Injecting Cells with Pulverized Fibers for Improved Cell Survival and Engraftment.Bio-protocol · 2024Article
- Adipose-Derived Stromal Cell-Based Therapies for Radiation-Induced Fibrosis.Advances in wound care · 2024Review
- In Vitro Characterization of Fat Grafts Processed Using the REVOLVE ENVI System versus Decantation.Plastic and reconstructive surgery. Global open · 2024Article
- Injectable pulverized electrospun poly(lactic-co-glycolic acid) fibers improve human adipose tissue engraftment and volume retention.Journal of biomedical materials research. Part A · 2023Article
- The preliminary study of exosomes derived from thymosin beta 4-treated adipose-derived stem cells in fat grafting.Genes & genomics · 2023Article
- Article
- Cell-Assisted Lipotransfer in Breast Augmentation Surgery: Clinical Outcomes and Considerations for Future Research.Cureus · 2022Review
- A Preliminary Exploratory Study of Autologous Fat Transplantation in Breast Augmentation With Different Fat Transplantation Planes.Frontiers in surgery · 2022Article
- Article
- Adipose-Derived Mesenchymal Stromal Cells in Regenerative Medicine: State of Play, Current Clinical Trials, and Future Prospects.Advances in wound care · 2021Review
- Human adipose-derived stem cells enriched with VEGF-modified mRNA promote angiogenesis and long-term graft survival in a fat graft transplantation model.Stem cell research & therapy · 2020Article
- CD34+CD146+ adipose-derived stromal cells enhance engraftment of transplanted fat.Stem cells translational medicine · 2020Article
- Fat grafts enriched with adipose-derived stem cells.Archives of craniofacial surgery · 2020Article
- Fat extract improves fat graft survival via proangiogenic, anti-apoptotic and pro-proliferative activities.Stem cell research & therapy · 2019Article
- Parry Romberg Syndrome: Literature Review and Report of Three Cases.Journal of maxillofacial and oral surgery · 2019Article
- Fat Grafting into Younger Recipients Improves Volume Retention in an Animal Model.Plastic and reconstructive surgery · 2019Article
- Fat Chance: The Rejuvenation of Irradiated Skin.Plastic and reconstructive surgery. Global open · 2019Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundFat graft volume retention remains highly unpredictable, but addition of adipose-derived stromal cells to fat grafts has been shown to improve retention. The present study aimed to investigate the mechanisms involved in adipose-derived stromal cell enhancement of fat grafting.
methodsAdipose-derived stromal cells isolated from human lipoaspirate were labeled with green fluorescent protein and luciferase. Fat grafts enhanced with adipose-derived stromal cells were injected into the scalp and bioluminescent imaging was performed to follow retention of adipose-derived stromal cells within the fat graft. Fat grafts were also explanted at days 1, 5, and 10 after grafting for adipose-derived stromal cell extraction and single-cell gene analysis. Finally, CD31 immunohistochemical staining was performed on fat grafts enriched with adipose-derived stromal cells.
resultsBioluminescent imaging demonstrated significant reduction in luciferase-positive adipose-derived stromal cells within fat grafts at 5 days after grafting. A similar reduction in viable green fluorescent protein-positive adipose-derived stromal cells retrieved from explanted grafts was also noted. Single-cell analysis revealed expression of multiple genes/markers related to cell survival and angiogenesis, including BMPR2, CD90, CD105, FGF2, CD248, TGFß1, and VEGFA. Genes involved in adipogenesis were not expressed by adipose-derived stromal cells. Finally, CD31 staining revealed significantly higher vascular density in fat grafts explanted at day 10 after grafting.
conclusionsAlthough adipose-derived stromal cell survival in the hypoxic graft environment decreases significantly over time, these cells provide multiple angiogenic growth factors. Therefore, improved fat graft volume retention with adipose-derived stromal cell enrichment may be attributable to improved graft vascularization.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.