Evidence map›Paper›PMID 25502569›Full record

ArticlePloS one2014

Cellular responses during morphological transformation in Azospirillum brasilense and Its flcA knockout mutant.

Xingsheng Hou, Mary McMillan, Joëlle V F Coumans, Anne Poljak, Mark J Raftery, Lily Pereg

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.1field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 21 citations in OpenAlex.

  1. Article
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  7. Maize Inoculation withFrontiers in microbiology · 2017
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Xingsheng HouSchool of Science and Technology, University of New England, Armidale, New South Wales, Australia; Department of Microbiology and Immunology, Shanxi Medical University, Taiyuan, Shanxi, China.
Mary McMillanSchool of Science and Technology, University of New England, Armidale, New South Wales, Australia.
Joëlle V F CoumansSchool of Science and Technology, University of New England, Armidale, New South Wales, Australia; School of Rural Medicine, University of New England, Armidale, New South Wales, Australia.
Anne PoljakBioanalytical Mass Spectrometry Facility, Analytical Centre, University of New South Wales, Sydney, New South Wales, Australia; The School of Medical Sciences, University of New South Wales, Sydney, New South Wales, Australia.
Mark J RafteryBioanalytical Mass Spectrometry Facility, Analytical Centre, University of New South Wales, Sydney, New South Wales, Australia.
Lily PeregSchool of Science and Technology, University of New England, Armidale, New South Wales, Australia.
University of New England · AUUNSW Sydney · AUShanxi Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

FlcA is a response regulator controlling flocculation and the morphological transformation of Azospirillum cells from vegetative to cyst-like forms. To understand the cellular responses of Azospirillum to conditions that cause morphological transformation, proteins differentially expressed under flocculation conditions in A. brasilense Sp7 and its flcA knockout mutant were investigated. Comparison of 2-DE protein profiles of wild-type (Sp7) and a flcA deletion mutant (Sp7-flcAΔ) revealed a total of 33 differentially expressed 2-DE gel spots, with 22 of these spots confidently separated to allow protein identification. Analysis of these spots by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and MASCOT database searching identified 48 proteins (≥10% emPAI in each spot). The functional characteristics of these proteins included carbon metabolism (beta-ketothiolase and citrate synthase), nitrogen metabolism (Glutamine synthetase and nitric oxide synthase), stress tolerance (superoxide dismutase, Alkyl hydroperoxidase and ATP-dependent Clp protease proteolytic subunit) and morphological transformation (transducer coupling protein). The observed differences between Sp7 wild-type and flcA- strains enhance our understanding of the morphological transformation process and help to explain previous phenotypical observations. This work is a step forward in connecting the Azospirillum phenome and genome.

Indexed as

Gene Knockout TechniquesMutationAzospirillum brasilenseBacterial ProteinsCarbohydrate MetabolismCongo RedFlocculationGene Expression ProfilingNitrogen FixationPhenotypePlant RootsProteomicsStress, PhysiologicalTranscription FactorsBacterial ProteinsCongo RedFlcA protein, Azospirillum brasilenseTranscription Factors

Identifiers

PMID25502569
PMCPMC4264754
OpenAlexW2087714525

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.