ArticleJournal of clinical microbiology2015
Accurate quantification of episomal HIV-1 two-long terminal repeat circles by use of optimized DNA isolation and droplet digital PCR.
Article in Journal of clinical microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Combination treatment with synthetic gRNA/Cas12a and gRNA/Cas9 ribonucleoproteins disrupts HIV replication and expression.iScience · 2026Article
- Undetectable unintegrated HIV-DNA in persons with HIV: frequency and determinants.BMC infectious diseases · 2026Article
- Validation of digital droplet PCR assays for cell-associated HIV-1 DNA, HIV-1 2-LTR circle, and HIV-1 unspliced RNA for clinical studies in HIV-1 cure research.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2024Article
- Accuracy of real-time PCR and digital PCR for the monitoring of total HIV DNA under prolonged antiretroviral therapy.Scientific reports · 2022Article
- A comparative analysis of unintegrated HIV-1 DNA measurement as a potential biomarker of the cellular reservoir in the blood of patients controlling and non-controlling viral replication.Journal of translational medicine · 2020Article
- Underestimated effect of intragenic HIV-1 DNA methylation on viral transcription in infected individuals.Clinical epigenetics · 2020Article
- Early treated HIV-1 positive individuals demonstrate similar restriction factor expression profile as long-term non-progressors.EBioMedicine · 2019Article
- Development of sensitive ddPCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant forms.Journal of the International AIDS Society · 2018Article
- Digital PCR as a tool to measure HIV persistence.Retrovirology · 2018Review
- Article
- Protein expression from unintegrated HIV-1 DNA introduces bias in primary in vitro post-integration latency models.Scientific reports · 2016Article
- The Future of Digital Polymerase Chain Reaction in Virology.Molecular diagnosis & therapy · 2016Review
- Virion encapsidated HIV-1 Vpr induces NFAT to prime non-activated T cells for productive infection.Open biology · 2016Article
- Integrated and Total HIV-1 DNA Predict Ex Vivo Viral Outgrowth.PLoS pathogens · 2016Article
- Aviremia 10 Years Postdiscontinuation of Antiretroviral Therapy Initiated During Primary Human Immunodeficiency Virus-1 Infection and Association With Gag-Specific T-Cell Responses.Open forum infectious diseases · 2015Article
- Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Episomal HIV-1 two-long terminal repeat (2-LTR) circles are considered markers for ongoing viral replication. Two sample processing procedures were compared to accurately quantify 2-LTR in patients by using droplet digital PCR (ddPCR). Here, we show that plasmid isolation with a spiked non-HIV plasmid for normalization enables more accurate 2-LTR quantification than genomic DNA isolation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.