ArticlePloS one2014
In vitro reconstitution of yeast tUTP/UTP A and UTP B subcomplexes provides new insights into their modular architecture.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Article
- Structural Probing with MNase Tethered to Ribosome Assembly Factors Resolves Flexible RNA Regions within the Nascent Pre-Ribosomal RNA.Non-coding RNA · 2022Article
- Anti-infective nitazoxanide disrupts transcription of ribosome biogenesis-related genes in yeast.Genes & genomics · 2020Article
- Pol5 is required for recycling of small subunit biogenesis factors and for formation of the peptide exit tunnel of the large ribosomal subunit.Nucleic acids research · 2020Article
- Ribosome assembly coming into focus.Nature reviews. Molecular cell biology · 2019Review
- TbUTP10, a protein involved in early stages of pre-18S rRNA processing in Trypanosoma brucei.Molecular and biochemical parasitology · 2018Article
- Assembly and structure of the SSU processome-a nucleolar precursor of the small ribosomal subunit.Current opinion in structural biology · 2018Review
- The SSU processome interactome inRNA (New York, N.Y.) · 2018Article
- 3.2-Å-resolution structure of the 90S preribosome before A1 pre-rRNA cleavage.Nature structural & molecular biology · 2017Article
- Pwp2 mediates UTP-B assembly via two structurally independent domains.Scientific reports · 2017Article
- Article
- Interaction network of the ribosome assembly machinery from a eukaryotic thermophile.Protein science : a publication of the Protein Society · 2017Article
- Structural basis for 5'-ETS recognition by Utp4 at the early stages of ribosome biogenesis.PloS one · 2017Article
- The Contributions of the Ribosome Biogenesis Protein Utp5/WDR43 to Craniofacial Development.Journal of dental research · 2016Review
- Integrative structural analysis of the UTPB complex, an early assembly factor for eukaryotic small ribosomal subunits.Nucleic acids research · 2016Article
- UtpA and UtpB chaperone nascent pre-ribosomal RNA and U3 snoRNA to initiate eukaryotic ribosome assembly.Nature communications · 2016Article
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6 authors.
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Abstract
Eukaryotic ribosome biogenesis is a multistep process involving more than 150 biogenesis factors, which interact transiently with pre-ribosomal particles to promote their maturation. Some of these auxiliary proteins have been isolated in complexes found separate from the ribosomal environment. Among them, are 3 large UTP subcomplexes containing 6 or 7 protein subunits which are involved in the early steps of ribosome biogenesis. The composition of the UTP subcomplexes and the network of binary interactions between protein subunits have been analyzed previously. To obtain further insights into the structural and biochemical properties of UTP subcomplexes, we established a heterologous expression system to allow reconstitution of the yeast tUTP/UTP A and UTP B subcomplexes from their candidate subunits. The results of a series of reconstitution experiments involving different combinations of protein subunits are in good agreement with most of the previously observed binary interactions. Moreover, in combination with additional biochemical analyses, several stable building blocks of the UTP subcomplexes were identified. Based on these findings, we present a refined model of the tUTP/UTP A and UTP B architecture.
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