ArticleJournal of cellular biochemistry2015
Quantitative superresolution microscopy reveals differences in nuclear DNA organization of multiple myeloma and monoclonal gammopathy of undetermined significance.
Article in Journal of cellular biochemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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Who cites it
11 citing papers in PubMed, 19 citations in OpenAlex.
- Article
- Review
- Multilevel view on chromatin architecture alterations in cancer.Frontiers in genetics · 2022Review
- Article
- Review
- p53 CRISPR Deletion Affects DNA Structure and Nuclear Architecture.Journal of clinical medicine · 2020Article
- High-resolution microscopy for imaging cancer pathobiology.Current pathobiology reports · 2019Article
- Super-resolution binding activated localization microscopy through reversible change of DNA conformation.Nucleus (Austin, Tex.) · 2018Review
- Super-resolution structure of DNA significantly differs in buccal cells of controls and Alzheimer's patients.Journal of cellular physiology · 2017Article
- Imaging chromatin nanostructure with binding-activated localization microscopy based on DNA structure fluctuations.Nucleic acids research · 2017Article
- Distinct and shared three-dimensional chromosome organization patterns in lymphocytes, monoclonal gammopathy of undetermined significance and multiple myeloma.International journal of cancer · 2017Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 6 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mammalian nucleus has a distinct substructure that cannot be visualized directly by conventional microscopy. In this study, the organization of the DNA within the nucleus of multiple myeloma (MM) cells, their precursor cells (monoclonal gammopathy of undetermined significance; MGUS) and control lymphocytes of the representative patients is visualized and quantified by superresolution microscopy. Three-dimensional structured illumination microscopy (3D-SIM) increases the spatial resolution beyond the limits of conventional widefield fluorescence microscopy. 3D-SIM reveals new insights into the nuclear architecture of cancer as we show for the first time that it resolves organizational differences in intranuclear DNA organization of myeloma cells in MGUS and in MM patients. In addition, we report a significant increase in nuclear submicron DNA structure and structure of the DNA-free space in myeloma nuclei compared to normal lymphocyte nuclei. Our study provides previously unknown details of the nanoscopic DNA architecture of interphase nuclei of the normal lymphocytes, MGUS and MM cells. This study opens new avenues to understanding the disease progression from MGUS to MM.
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Registered trials
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