ArticleApplied and environmental microbiology2015
Role of plasmids in Lactobacillus brevis BSO 464 hop tolerance and beer spoilage.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 35 citations in OpenAlex.
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- Depiction of the In Vitro and Genomic Basis of Resistance to Hop and High Hydrostatic Pressure ofGenes · 2023Article
- Characterization and sequencing analysis of pLP2.5-11 and pLP3.0-4 novel cryptic plasmids from3 Biotech · 2023Article
- Identification of Type II Toxin-Antitoxin Loci in Levilactobacillus brevis.Interdisciplinary sciences, computational life sciences · 2022Article
- Draft Genome Sequence of Lactiplantibacillus plantarum NMZ-1139, Isolated from Whisky Mash.Microbiology resource announcements · 2021Article
- Microbial Dynamics in Traditional and Modern Sour Beer Production.Applied and environmental microbiology · 2020Review
- A Plasmid-Encoded Putative Glycosyltransferase Is Involved in Hop Tolerance and Beer Spoilage in Lactobacillus brevis.Applied and environmental microbiology · 2020Article
- Co-fermentation InvolvingFrontiers in microbiology · 2020Article
- Heterogeneity between and within Strains ofFrontiers in microbiology · 2017Article
- Multiple Genome Sequences of Important Beer-Spoiling Lactic Acid Bacteria.Genome announcements · 2016Article
- Article
- Genome Sequence of Rapid Beer-Spoiling Isolate Lactobacillus brevis BSO 464.Genome announcements · 2015Article
- Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Specific isolates of lactic acid bacteria (LAB) can grow in the harsh beer environment, thus posing a threat to brew quality and the economic success of breweries worldwide. Plasmid-localized genes, such as horA, horC, and hitA, have been suggested to confer hop tolerance, a trait required for LAB survival in beer. The presence and expression of these genes among LAB, however, do not universally correlate with the ability to grow in beer. Genome sequencing of the virulent beer spoilage organism Lactobacillus brevis BSO 464 revealed the presence of eight plasmids, with plasmids 1, 2, and 3 containing horA, horC, and hitA, respectively. To investigate the roles that these and the other five plasmids play in L. brevis BSO 464 growth in beer, plasmid curing with novobiocin was used to derive 10 plasmid variants. Multiplex PCRs were utilized to determine the presence or absence of each plasmid, and how plasmid loss affected hop tolerance and growth in degassed (noncarbonated) beer was assessed. Loss of three of the eight plasmids was found to affect hop tolerance and growth in beer. Loss of plasmid 2 (horC and 28 other genes) had the most dramatic effect, with loss of plasmid 4 (120 genes) and plasmid 8 (47 genes) having significant, but smaller, impacts. These results support the contention that genes on mobile genetic elements are essential for bacterial growth in beer and that beer spoilage ability is not dependent solely on the three previously described hop tolerance genes or on the chromosome of a beer spoilage LAB isolate.
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