ArticlePLoS genetics2014
Non-coding roX RNAs prevent the binding of the MSL-complex to heterochromatic regions.
Article in PLoS genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 32 citations in OpenAlex.
- Dosage compensation defects due to roX RNA loss are rescued by recalibration of X/autosome stoichiometry.Nucleic acids research · 2026Article
- Dynamics and regulatory roles of RNA meLife · 2025Article
- A noncanonical role of roX RNAs in autosomal epigenetic repression.Nature communications · 2025Article
- Physical interaction between MSL2 and CLAMP assures direct cooperativity and prevents competition at composite binding sites.Nucleic acids research · 2023Article
- Dosage Compensation inInternational journal of molecular sciences · 2022Review
- Review
- The role of H3K36 methylation and associated methyltransferases in chromosome-specific gene regulation.Science advances · 2021Article
- Divergent evolution toward sex chromosome-specific gene regulation inGenes & development · 2021Article
- Article
- Rapid Evolution of Autosomal Binding Sites of the Dosage Compensation Complex inFrontiers in genetics · 2021Article
- Two-step mechanism for selective incorporation of lncRNA into a chromatin modifier.Nucleic acids research · 2020Article
- Faint gray bands in Drosophila melanogaster polytene chromosomes are formed by coding sequences of housekeeping genes.Chromosoma · 2020Article
- Article
- Structure, dynamics and roX2-lncRNA binding of tandem double-stranded RNA binding domains dsRBD1,2 of Drosophila helicase Maleless.Nucleic acids research · 2019Article
- lncRedibly versatile: biochemical and biological functions of long noncoding RNAs.The Biochemical journal · 2019Review
- RNA-on-X 1 and 2 in Drosophila melanogaster fulfill separate functions in dosage compensation.PLoS genetics · 2018Article
- TheGenetics · 2018Article
- Facultative dosage compensation of developmental genes on autosomes in Drosophila and mouse embryonic stem cells.Nature communications · 2018Article
- Article
- Primary Sex Determination in Drosophila melanogaster Does Not Rely on the Male-Specific Lethal Complex.Genetics · 2016Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Long non-coding RNAs contribute to dosage compensation in both mammals and Drosophila by inducing changes in the chromatin structure of the X-chromosome. In Drosophila melanogaster, roX1 and roX2 are long non-coding RNAs that together with proteins form the male-specific lethal (MSL) complex, which coats the entire male X-chromosome and mediates dosage compensation by increasing its transcriptional output. Studies on polytene chromosomes have demonstrated that when both roX1 and roX2 are absent, the MSL-complex becomes less abundant on the male X-chromosome and is relocated to the chromocenter and the 4th chromosome. Here we address the role of roX RNAs in MSL-complex targeting and the evolution of dosage compensation in Drosophila. We performed ChIP-seq experiments which showed that MSL-complex recruitment to high affinity sites (HAS) on the X-chromosome is independent of roX and that the HAS sequence motif is conserved in D. simulans. Additionally, a complete and enzymatically active MSL-complex is recruited to six specific genes on the 4th chromosome. Interestingly, our sequence analysis showed that in the absence of roX RNAs, the MSL-complex has an affinity for regions enriched in Hoppel transposable elements and repeats in general. We hypothesize that roX mutants reveal the ancient targeting of the MSL-complex and propose that the role of roX RNAs is to prevent the binding of the MSL-complex to heterochromatin.
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