ArticleClinical and experimental nephrology2015
pH-sensitive expression of calcium-sensing receptor (CaSR) in type-B intercalated cells of the cortical collecting ducts (CCD) in mouse kidney.
Article in Clinical and experimental nephrology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed, 15 citations in OpenAlex.
- Effects of Roxadustat on Erythropoietin Production in the Rat Body.Molecules (Basel, Switzerland) · 2022Article
- Effects of Angiotensin II on Erythropoietin Production in the Kidney and Liver.Molecules (Basel, Switzerland) · 2021Article
- Thy1 promoter activity in the Rosa26 locus in mice: lessons from Dre-rox conditional expression system.Experimental animals · 2020Article
- Erythropoietin production by the kidney and the liver in response to severe hypoxia evaluated by Western blotting with deglycosylation.Physiological reports · 2020Article
- Intercalated Cells of the Kidney Collecting Duct in Kidney Physiology.Seminars in nephrology · 2019Review
- Calcium-sensing receptor: evidence and hypothesis for its role in nephrolithiasis.Urolithiasis · 2019Review
- Calcium-sensing receptor (CaSR) modulates vacuolar HClinical and experimental nephrology · 2018Article
- Renal Tubular Acidosis: HAdvances in chronic kidney disease · 2018Review
- Comparative expression of the extracellular calcium-sensing receptor in the mouse, rat, and human kidney.American journal of physiology. Renal physiology · 2016Article
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5 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundThe localization and role of the calcium-sensing receptor (CaSR) along the nephron including the collecting ducts is still open to debate.
methodsUsing the quantitative, highly sensitive in situ hybridization technique and a double-staining immunohistochemistry technique, we investigated the axial distribution and expression of CaSR along the nephron in mice (C57B/6J) treated for 6 days with acid or alkali diets.
resultsUnder control condition, CaSR was specifically localized in the cortical and medullary thick ascending limb of Henle's loop (CTAL and MTAL), macula densa (MD), distal convoluted tubule (DCT), and CCD (TALs, MD > DCT, CCD). Along the CCD, CaSR was co-localized with an anion exchanger type 4 (AE4), a marker of the basolateral membrane of type-B intercalated cell (IC-B) in mice. On the contrary, CaSR was not detected either in principal cells (PC) or in type-A intercalated cell (IC-A). CaSR expression levels in IC-B significantly (P < 0.005) decreased when mice were fed NH4Cl (acid) diets and increased when animals were given NaHCO3 (alkali) diets. As expected, cell heights of IC-A and IC-B significantly (P < 0.005) increased in the above experimental conditions. Surprisingly, single infusion (ip) of neomycin, an agonist of CaSR, significantly (P < 0.005) increased urinary Ca excretion without further increasing the hourly urine volume and significantly (P < 0.05) decreased urine pH.
conclusionCaSR, cloned from rat kidney, was localized in the basolateral membrane of IC-B and was more expressed during alkali-loading. Its alkali-sensitive expression may promote urinary alkali secretion for body acid-base balance.
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