ArticleJournal of experimental & clinical cancer research : CR2014
Overexpression of brachyury contributes to tumor metastasis by inducing epithelial-mesenchymal transition in hepatocellular carcinoma.
Article in Journal of experimental & clinical cancer research : CR, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 67 citations in OpenAlex.
- Trial
- Sublethal heat stress synergizes with the tumor microenvironment to drive recurrence of hepatocellular carcinoma after thermal ablation: mechanisms, molecular predictors, and targeted interventions.Cell biology and toxicology · 2026Review
- A single-cell and tissue-scale analysis suite resolves Mixl1's role in heart development.iScience · 2025Article
- Effect of β-catenin on hypoxia induced epithelial mesenchymal transition in HK-2 cells by regulating Brachyury.Biochemistry and biophysics reports · 2025Article
- Design of an In Vitro Model for Epithelial-to-Mesenchymal Transition in Gastric Cancer.Biochemical genetics · 2025Article
- Context-dependent T-BOX transcription factor family: from biology to targeted therapy.Cell communication and signaling : CCS · 2024Review
- Basal delamination during mouse gastrulation primes pluripotent cells for differentiation.Developmental cell · 2024Article
- Immunotherapy, targeted therapy, and their cross talks in hepatocellular carcinoma.Frontiers in immunology · 2023Review
- Brachyury promotes proliferation and migration of hepatocellular carcinoma via facilitating the transcription of NCAPG2.American journal of cancer research · 2022Article
- Multiple malignant tumors in a patient with familial chordoma, a case report.BMC medical genomics · 2021Article
- miR-219-5p targets TBXT and inhibits breast cancer cell EMT and cell migration and invasion.Bioscience reports · 2021Article
- Loss of MGA repression mediated by an atypical polycomb complex promotes tumor progression and invasiveness.eLife · 2021Article
- Aldehyde Dehydrogenase, a Therapeutic Target in Chordoma: Analysis in 3D Cellular Models.Cells · 2021Article
- The association of lncRNA SNPs and SNPs-environment interactions based on GWAS with HBV-related HCC risk and progression.Molecular genetics & genomic medicine · 2021Article
- The Roles of Embryonic Transcription Factor BRACHYURY in Tumorigenesis and Progression.Frontiers in oncology · 2020Review
- Identification of the Different Roles and Potential Mechanisms of T Isoforms in the Tumor Recurrence and Cell Cycle of Chordomas.OncoTargets and therapy · 2019Article
- BTNET : boosted tree based gene regulatory network inference algorithm using time-course measurement data.BMC systems biology · 2018Article
- UM-Chor1: establishment and characterization of the first validated clival chordoma cell line.Journal of neurosurgery · 2018Article
- M3 muscarinic acetylcholine receptors regulate epithelial-mesenchymal transition, perineural invasion, and migration/metastasis in cholangiocarcinoma through the AKT pathway.Cancer cell international · 2018Article
- Noncoding RNA Transcripts during Differentiation of Induced Pluripotent Stem Cells into Hepatocytes.Stem cells international · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsBrachyury overexpression has been reported in various human malignant neoplasms, but its expression and function in hepatocellular carcinoma progression and metastasis remains unknown. The present study aimed to evaluate the critical role of Brachyury in HCC metastasis.
methodsThe expression of Brachyury in human HCC (SMMC7721, HepG2, FHCC98, and Hep3B) and control cell lines was analyzed using quantitative reverse-transcriptase polymerase chain reaction and immunoflourence methods. Cancerous tissues collected from patients with HCC (n = 112) were analyzed using immunohistochemical method; a microarray analysis of HCC tissues was performed to explore the clinicopathological variables of HCC. The migratory and invasive capacities of Brachyury-SMMC7721 and Brachyury-HepG2 transfected cells were evaluated using in vitro scratch wound healing and Matrigel invasion assays, respectively. Further, six-week-old male BALB/c nude mice (n = 10) model was used in vivo assay.
resultsElevated expression of Brachyury was detected in HCCs (62.5%) compared with that in adjacent nontumorous tissues. Clinicopathological analysis revealed a close correlation of Brachyury expression with distant metastasis and poor prognosis of HCC. Overexpression of Brachyury promoted epithelial-mesenchymal transition (EMT) and metastasis of HCC cells in vitro and in vivo. Brachyury overexpression enhanced Akt activation by inhibiting phosphatase and tensin homolog (PTEN), which led to subsequent stabilization of Snail, a critical EMT mediator.
conclusionThe study findings suggest that elevated Brachyury facilitates HCC metastasis by promoting EMT via PTEN/Akt/Snail-dependent pathway. Brachyury plays a pivotal role in HCC metastasis and may serve as a novel prognostic biomarker and therapeutic target.
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