ArticleParasites & vectors2014
DNA barcoding: complementing morphological identification of mosquito species in Singapore.
Article in Parasites & vectors, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 92 papers, 1 of them a synthesis that pooled it.
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Who cites it
92 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Meta-analyses of Culex blood-meals indicates strong regional effect on feeding patterns.PLoS neglected tropical diseases · 2025Pooled it
- From RAMP to Triplex RT-qPCR: Modernizing Arbovirus Surveillance and Confirming the FirstPathogens (Basel, Switzerland) · 2026Article
- Genetic Diversity and Phylogenetic Relationships of Mosquitoes (Diptera: Culicidae) in Central Nepal.Ecology and evolution · 2026Article
- In silico DNA barcoding surpasses whole genome sequencing for species identification from vector surveillance pools.Scientific reports · 2026Article
- Different larval habitats and insecticide resistance frequency of Anopheles sinensis in Ganghwagun, Incheon, South Korea.Scientific reports · 2025Article
- Bulk identification of Anophelinae larvae from fishponds using DNA metabarcoding.Acta tropica · 2025Article
- Barcoding Quantitative PCR Assay to Distinguish BetweenTropical medicine and infectious disease · 2025Article
- The Frontier of Entomo-Virology: Applications and Tools for Virus and Vector Surveillance.Pathogens (Basel, Switzerland) · 2025Review
- Molecular Survey and Genetic Identification of Wolbachia Endosymbionts in Dwelling-Caught Culex quinquefasciatus (Diptera: Culicidae) Mosquitoes from Taiwan.Microbial ecology · 2025Article
- Mitogenomes of mosquito species of Harris County in Texas.Scientific reports · 2025Article
- Redefining the Geographic Distribution of Two CrypticEcology and evolution · 2025Article
- The ecological niche and population history shape mosquito population genetics on a group of three Caribbean islands.Parasites & vectors · 2025Article
- Real-time dynamic monitoring and multiplex PCR identification of vector mosquitoes in Zhejiang, China.PLoS neglected tropical diseases · 2025Article
- COI Insights into Diversity and Species Delimitation of Immature Stages of Non-Biting Midges (Diptera: Chironomidae).Insects · 2025Article
- The application of DNA markers in population genetics of mosquitoes: a comprehensive review.Frontiers in insect science · 2025Review
- Article
- Extensive surveillance of mosquitoes and molecular investigation of arboviruses in Central Iran.Annals of medicine and surgery (2012) · 2025Article
- DNA Barcoding for the Identification of Adult Mosquitoes (Diptera: Culicidae) in Western Australia.Ecology and evolution · 2024Article
- Species identification of livefood flightless fly (Ecology and evolution · 2024Article
- Review
32 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTaxonomy that utilizes morphological characteristics has been the gold standard method to identify mosquito species. However, morphological identification is challenging when the expertise is limited and external characters are damaged because of improper specimen handling. Therefore, we explored the applicability of mitochondrial cytochrome C oxidase subunit 1 (COI) gene-based DNA barcoding as an alternative tool to identify mosquito species. In the present study, we compared the morphological identification of mosquito specimens with their differentiation based on COI barcode, in order to establish a more reliable identification system for mosquito species found in Singapore.
methodsWe analysed 128 adult mosquito specimens, belonging to 45 species of 13 genera. Phylogenetic trees were constructed for Aedes, Anopheles, Culex and other genera of mosquitoes and the distinctive clustering of different species was compared with their taxonomic identity.
resultsThe COI-based DNA barcoding achieved a 100% success rate in identifying the mosquito species. We also report COI barcode sequences of 16 mosquito species which were not available previously in sequence databases.
conclusionsOur study utilised for the first time DNA barcoding to identify mosquito species in Singapore. COI-based DNA barcoding is a useful tool to complement taxonomy-based identification of mosquito species.
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