Evidence map›Paper›PMID 25497303›Full record

ArticleParticle and fibre toxicology2014

Single-walled carbon nanotubes increase pandemic influenza A H1N1 virus infectivity of lung epithelial cells.

Pallab Sanpui, Xiao Zheng, Julia C Loeb, Joseph H Bisesi, Iftheker A Khan, A R M Nabiul Afrooz, Keira Liu, Appala Raju Badireddy, Mark R Wiesner, P Lee Ferguson and 3 more

Open access · goldAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Particle and fibre toxicology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed, 1 pooled it
2.0field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 1 synthesis or guideline pooled it, 50 citations in OpenAlex.

  1. Pooled it
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  5. A Narrative Review of Occupational Air Pollution and Respiratory Health in Farmworkers.International journal of environmental research and public health · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 1 country.

Pallab SanpuiDepartment of Environmental and Global Health, Center for Environmental and Human Toxicology and Emerging Pathogens Institute, University of Florida, 2187 Mowry Road, Box 110885, Gainesville, FL, 32611, USA. pallabsanpui@gmail.com.
Xiao ZhengDepartment of Environmental and Global Health, Center for Environmental and Human Toxicology and Emerging Pathogens Institute, University of Florida, 2187 Mowry Road, Box 110885, Gainesville, FL, 32611, USA. xiaozhengxjtu@gmail.com.
Julia C LoebDepartment of Environmental and Global Health, Center for Environmental and Human Toxicology and Emerging Pathogens Institute, University of Florida, 2187 Mowry Road, Box 110885, Gainesville, FL, 32611, USA. jloeb@phhp.ufl.edu.
Joseph H BisesiDepartment of Environmental and Global Health, Center for Environmental and Human Toxicology and Emerging Pathogens Institute, University of Florida, 2187 Mowry Road, Box 110885, Gainesville, FL, 32611, USA. jbisesi@phhp.ufl.edu.
Iftheker A KhanDepartment of Civil, Architectural and Environmental Engineering, University of Texas at Austin, 301 E. Dean Keeton Street, Austin, TX, 78712, USA. iftheker04@gmail.com.
A R M Nabiul AfroozDepartment of Civil, Architectural and Environmental Engineering, University of Texas at Austin, 301 E. Dean Keeton Street, Austin, TX, 78712, USA. rnabiul@gmail.com.
Keira LiuDepartment of Civil and Environmental Engineering, Nicholas School of the Environment, and Center for the Environmental Implications of NanoTechnology, Duke University, 121 Hudson Hall, Box 90287, Durham, NC, 27708, USA. keiraliu125@gmail.com.
Appala Raju BadireddyDepartment of Civil and Environmental Engineering, Nicholas School of the Environment, and Center for the Environmental Implications of NanoTechnology, Duke University, 121 Hudson Hall, Box 90287, Durham, NC, 27708, USA. appala.badireddy@duke.edu.
Mark R WiesnerDepartment of Civil and Environmental Engineering, Nicholas School of the Environment, and Center for the Environmental Implications of NanoTechnology, Duke University, 121 Hudson Hall, Box 90287, Durham, NC, 27708, USA. wiesner@duke.edu.
P Lee FergusonDepartment of Civil and Environmental Engineering, Nicholas School of the Environment, and Center for the Environmental Implications of NanoTechnology, Duke University, 121 Hudson Hall, Box 90287, Durham, NC, 27708, USA. lee.ferguson@duke.edu.
Navid B SalehDepartment of Civil, Architectural and Environmental Engineering, University of Texas at Austin, 301 E. Dean Keeton Street, Austin, TX, 78712, USA. navid.saleh@utexas.edu.
John A LednickyDepartment of Environmental and Global Health, Center for Environmental and Human Toxicology and Emerging Pathogens Institute, University of Florida, 2187 Mowry Road, Box 110885, Gainesville, FL, 32611, USA. jlednicky@phhp.ufl.edu.
Tara Sabo-AttwoodDepartment of Environmental and Global Health, Center for Environmental and Human Toxicology and Emerging Pathogens Institute, University of Florida, 2187 Mowry Road, Box 110885, Gainesville, FL, 32611, USA. sabo@phhp.ufl.edu.
University of Florida · USDuke University · USThe University of Texas at Austin · US

Funding

Contribution of Toll-like Receptors in the Pulmonary Response to Nanoparticles anR01HL114907 · NHLBI · UNIVERSITY OF FLORIDA · PI SABO-ATTWOOD, TARA L · 2012 to 2016
$1.5M
NHLBI NIH HHS R01 HL114907NHLBI NIH HHS R01HL114907
6 · The paper itself

Abstract

backgroundAirborne exposure to nanomaterials from unintended occupational or environmental exposures or as a consequence of product use may lead to adverse health effects. Numerous studies have focused on single-walled carbon nanotubes (SWCNTs) and their ability to cause pulmonary injury related to fibrosis, and cancer; however few studies have addressed their impact on infectious agents, particularly viruses that are known for causing severe disease. Here we have demonstrated the ability of pristine SWCNTs of diverse electronic structure to increase the susceptibility of small airway epithelial cells (SAEC) to pandemic influenza A H1N1 infection and discerned potential mechanisms of action driving this response.

methodsSmall airway epithelial cells (SAEC) were exposed to three types of SWCNTs with varying electronic structure (SG65, SG76, CG200) followed by infection with A/Mexico/4108/2009 (pH1N1). Cells were then assayed for viral infectivity by immunofluorescence and viral titers. We quantified mRNA and protein levels of targets involved in inflammation and anti-viral activity (INFβ1, IL-8, RANTES/CCL5, IFIT2, IFIT3, ST3GAL4, ST6GAL1, IL-10), localized sialic acid receptors, and assessed mitochondrial function. Hyperspectral imaging analysis was performed to map the SWCNTs and virus particles in fixed SAEC preparations. We additionally performed characterization analysis to monitor SWCNT aggregate size and structure under biological conditions using dynamic light scattering (DLS), static light scattering (SLS).

resultsBased on data from viral titer and immunofluorescence assays, we report that pre-treatment of SAEC with SWCNTs significantly enhances viral infectivity that is not dependent on SWCNT electronic structure and aggregate size within the range of 106 nm - 243 nm. We further provide evidence to support that this noted effect on infectivity is not likely due to direct interaction of the virus and nanoparticles, but rather a combination of suppression of pro-inflammatory (RANTES) and anti-viral (IFIT2, IFIT3) gene/protein expression, impaired mitochondrial function and modulation of viral receptors by SWCNTs.

conclusionsResults of this work reveal the potential for SWCNTs to increase susceptibility to viral infections as a mechanism of adverse effect. These data highlight the importance of investigating the ability of carbon-nanomaterials to modulate the immune system, including impacts on anti-viral mechanisms in lung cells, thereby increasing susceptibility to infectious agents.

Indexed as

Air PollutantsApoptosis Regulatory ProteinsBronchiCells, CulturedCell SurvivalChemokine CCL5Gene Expression RegulationGene Expression Regulation, ViralHumansImmunity, InnateInfluenza A Virus, H1N1 SubtypeIntracellular Signaling Peptides and ProteinsMitochondriaNanotubes, CarbonParticle SizeProteinsAir PollutantsApoptosis Regulatory ProteinsCCL5 protein, humanChemokine CCL5IFIT2 protein, humanIFIT3 protein, humanIntracellular Signaling Peptides and ProteinsNanotubes, CarbonProteinsRNA-Binding Proteins

Identifiers

PMID25497303
PMCPMC4318452
OpenAlexW2119229440

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.