ArticleJournal of the American Chemical Society2014
Bioorthogonal tetrazine-mediated transfer reactions facilitate reaction turnover in nucleic acid-templated detection of microRNA.
Article in Journal of the American Chemical Society, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
43 citing papers in PubMed, 146 citations in OpenAlex.
- Enhanced through-bond energy transfer-based bioorthogonal probe enables catalytic-amplified and sensitive detection of microRNA-21 for clinical lung cancer diagnosis.Smart molecules : open access · 2026Article
- Biocompatible Chemistry: A Plug-and-Play Toolbox for Chemical Biology Research.Chembiochem : a European journal of chemical biology · 2025Review
- Inverted Molecular Beacons as Reaction-Based Hybridization Probes for Small-Molecule Activation by Nucleic Acid Inputs.ACS chemical biology · 2025Article
- Chemical autoligation with phosphorothioate- and sulfonamide-terminated DNA via intramolecular cross-activation.Communications chemistry · 2025Article
- Click-Triggered Bioorthogonal Bond-Cleavage Reactions.Topics in current chemistry (Cham) · 2025Review
- Fluorogenic Tetrazine Bioorthogonal Probes for Advanced Application in Bioimaging and Biomedicine.Chemical & biomedical imaging · 2025Article
- Late-Stage Functionalization of Living Organisms: Rethinking Selectivity in Biology.Chemical reviews · 2024Review
- Double Click: Unexpected 1:2 Stoichiometry in a Norbornene-Tetrazine Reaction.The Journal of organic chemistry · 2023Article
- Tetrazine-Induced Bioorthogonal Activation of Vitamin E-Modified siRNA for Gene Silencing.Molecules (Basel, Switzerland) · 2022Article
- [4 + 2] and [2 + 4] cycloaddition reactions on single- and double-stranded DNA: a dual-reactive nucleoside.RSC chemical biology · 2022Article
- Expanding the scope of native chemical ligation - templated small molecule drug synthesisChemical science · 2021Article
- A Self-immolative Molecular Beacon for Amplified Nucleic Acid Detection*.Chemistry (Weinheim an der Bergstrasse, Germany) · 2021Article
- Research Progress and Application of Bioorthogonal Reactions in Biomolecular Analysis and Disease Diagnosis.Topics in current chemistry (Cham) · 2021Review
- Structurally Redesigned Bioorthogonal Reagents for Mitochondria-Specific Prodrug Activation.JACS Au · 2021Article
- Concise Synthesis of Functionalized Cyclobutene Analogues for Bioorthogonal Tetrazine Ligation.Molecules (Basel, Switzerland) · 2021Article
- Covalent labeling of nucleic acids.Chemical Society reviews · 2020Review
- Activation and Delivery of Tetrazine-Responsive Bioorthogonal Prodrugs.Molecules (Basel, Switzerland) · 2020Review
- Nucleic Acid Conformation Influences Postsynthetic Suzuki-Miyaura Labeling of Oligonucleotides.Bioconjugate chemistry · 2020Article
- Visible-light triggered templated ligation on surface using furan-modified PNAs.Chemical science · 2020Article
- Labelling of DNA and RNA in the cellular environment by means of bioorthogonal cycloaddition chemistry.RSC chemical biology · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Tetrazine ligations have proven to be a powerful bioorthogonal technique for the detection of many labeled biomolecules, but the ligating nature of these reactions can limit reaction turnover in templated chemistry. We have developed a transfer reaction between 7-azabenzonorbornadiene derivatives and fluorogenic tetrazines that facilitates turnover amplification of the fluorogenic response in nucleic acid-templated reactions. Fluorogenic tetrazine-mediated transfer (TMT) reaction probes can be used to detect DNA and microRNA (miRNA) templates to 0.5 and 5 pM concentrations, respectively. The endogenous oncogenic miRNA target mir-21 could be detected in crude cell lysates and detected by imaging in live cells. Remarkably, the technique is also able to differentiate between miRNA templates bearing a single mismatch with high signal to background. We imagine that TMT reactions could find wide application for amplified fluorescent detection of clinically relevant nucleic acid templates.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.