ArticleAnalytical chemistry2015
Amphipols outperform dodecylmaltoside micelles in stabilizing membrane protein structure in the gas phase.
Article in Analytical chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 52 citations in OpenAlex.
- Infrared Photoactivation Enables Improved Native Top-Down Mass Spectrometry of Transmembrane Proteins.Analytical chemistry · 2023Article
- Nonionic, Cleavable Surfactant for Top-Down Proteomics.Analytical chemistry · 2023Article
- High-Throughput Native Mass Spectrometry Screening in Drug Discovery.Frontiers in molecular biosciences · 2022Review
- A Diazirine-Modified Membrane Lipid to Study Peptide/Lipid Interactions - Chances and Challenges.Chemistry (Weinheim an der Bergstrasse, Germany) · 2021Article
- Liposomes as Carriers of Membrane-Associated Proteins and Peptides for Mass Spectrometric Analysis.Angewandte Chemie (International ed. in English) · 2021Article
- Ion mobility-mass spectrometry reveals the role of peripheral myelin protein dimers in peripheral neuropathy.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Dendritic Oligoglycerol Regioisomer Mixtures and Their Utility for Membrane Protein Research.Chemistry (Weinheim an der Bergstrasse, Germany) · 2021Article
- The importance of the membrane for biophysical measurements.Nature chemical biology · 2020Review
- Collision-Induced Unfolding Differentiates Functional Variants of the KCNQ1 Voltage Sensor Domain.Journal of the American Society for Mass Spectrometry · 2020Article
- Styrene maleic-acid lipid particles (SMALPs) into detergent or amphipols: An exchange protocol for membrane protein characterisation.Biochimica et biophysica acta. Biomembranes · 2020Article
- Modular detergents tailor the purification and structural analysis of membrane proteins including G-protein coupled receptors.Nature communications · 2020Article
- Interrogating Membrane Protein Structure and Lipid Interactions by Native Mass Spectrometry.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Expanding the Types of Lipids Amenable to Native Mass Spectrometry of Lipoprotein Complexes.Journal of the American Society for Mass Spectrometry · 2019Article
- Fourier Transform-Ion Cyclotron Resonance Mass Spectrometry as a Platform for Characterizing Multimeric Membrane Protein Complexes.Journal of the American Society for Mass Spectrometry · 2018Article
- Topological Dissection of the Membrane Transport Protein Mhp1 Derived from Cysteine Accessibility and Mass Spectrometry.Analytical chemistry · 2017Article
- Ion Mobility Collision Cross Section Compendium.Analytical chemistry · 2017Review
- FPOP-LC-MS/MS Suggests Differences in Interaction Sites of Amphipols and Detergents with Outer Membrane Proteins.Journal of the American Society for Mass Spectrometry · 2017Article
- Interfacing Membrane Mimetics with Mass Spectrometry.Accounts of chemical research · 2016Article
- Skp is a multivalent chaperone of outer-membrane proteins.Nature structural & molecular biology · 2016Article
- Unraveling the Composition and Behavior of Heterogeneous Lipid Nanodiscs by Mass Spectrometry.Analytical chemistry · 2016Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Noncovalent mass spectrometry (MS) is emerging as an invaluable technique to probe the structure, interactions, and dynamics of membrane proteins (MPs). However, maintaining native-like MP conformations in the gas phase using detergent solubilized proteins is often challenging and may limit structural analysis. Amphipols, such as the well characterized A8-35, are alternative reagents able to maintain the solubility of MPs in detergent-free solution. In this work, the ability of A8-35 to retain the structural integrity of MPs for interrogation by electrospray ionization-ion mobility spectrometry-mass spectrometry (ESI-IMS-MS) is compared systematically with the commonly used detergent dodecylmaltoside. MPs from the two major structural classes were selected for analysis, including two β-barrel outer MPs, PagP and OmpT (20.2 and 33.5 kDa, respectively), and two α-helical proteins, Mhp1 and GalP (54.6 and 51.7 kDa, respectively). Evaluation of the rotationally averaged collision cross sections of the observed ions revealed that the native structures of detergent solubilized MPs were not always retained in the gas phase, with both collapsed and unfolded species being detected. In contrast, ESI-IMS-MS analysis of the amphipol solubilized MPs studied resulted in charge state distributions consistent with less gas phase induced unfolding, and the presence of lowly charged ions which exhibit collision cross sections comparable with those calculated from high resolution structural data. The data demonstrate that A8-35 can be more effective than dodecylmaltoside at maintaining native MP structure and interactions in the gas phase, permitting noncovalent ESI-IMS-MS analysis of MPs from the two major structural classes, while gas phase dissociation from dodecylmaltoside micelles leads to significant gas phase unfolding, especially for the α-helical MPs studied.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.