ArticleThe Plant journal : for cell and molecular biology2015
The maize brown midrib4 (bm4) gene encodes a functional folylpolyglutamate synthase.
Article in The Plant journal : for cell and molecular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 28 papers.
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28 citing papers in PubMed, 56 citations in OpenAlex.
- Insights into the genetic and biochemical basis of Gibberella ear rot resistance in maize.The plant genome · 2025Article
- Modulation of lignin and anthocyanin homeostasis by GTP cyclohydrolase1 in maize.Plant biotechnology journal · 2025Article
- Engineered reduction of S-adenosylmethionine alters lignin in sorghum.Biotechnology for biofuels and bioproducts · 2024Article
- Synthetic-biology approach for plant lignocellulose engineering.Plant biotechnology (Tokyo, Japan) · 2024Article
- Genome-Wide Identification and Characterization of Lignin Synthesis Genes in Maize.International journal of molecular sciences · 2024Article
- Breeding for improved digestibility and processing of lignocellulosic biomass inFrontiers in plant science · 2024Review
- Brown midrib mutant and genome-wide association analysis uncover lignin genes for disease resistance in maize.The plant genome · 2023Article
- Genetic structure and molecular mechanism underlying the stalk lodging traits in maize (Computational and structural biotechnology journal · 2023Article
- Article
- Characteristics and candidate genes associated with excellent stalk strength in maize (Frontiers in plant science · 2022Article
- Whole-genome analysis ofPeerJ · 2022Article
- The 5-formyl-tetrahydrofolate proteome links folates with C/N metabolism and reveals feedback regulation of folate biosynthesis.The Plant cell · 2021Article
- Pathogen and drought stress affect cell wall and phytohormone signaling to shape host responses in a sorghum COMT bmr12 mutant.BMC plant biology · 2021Article
- SorghumGenes · 2021Article
- The Sorghum (Frontiers in plant science · 2021Article
- SilencingFrontiers in plant science · 2020Article
- Characterization of Two NewFrontiers in plant science · 2020Article
- Multi-omics analysis of the development and fracture resistance for maize internode.Scientific reports · 2019Article
- Combining loss of function ofBiotechnology for biofuels · 2019Article
- Mutation of 4-coumarate: coenzyme A ligase 1 gene affects lignin biosynthesis and increases the cell wall digestibility in maizeBiotechnology for biofuels · 2019Article
Corrections and comments
- Erratum issuedCorrection.2017
Authors and funding
8 authors at 1 institution in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mutations in the brown midrib4 (bm4) gene affect the accumulation and composition of lignin in maize. Fine-mapping analysis of bm4 narrowed the candidate region to an approximately 105 kb interval on chromosome 9 containing six genes. Only one of these six genes, GRMZM2G393334, showed decreased expression in mutants. At least four of 10 Mu-induced bm4 mutant alleles contain a Mu insertion in the GRMZM2G393334 gene. Based on these results, we concluded that GRMZM2G393334 is the bm4 gene. GRMZM2G393334 encodes a putative folylpolyglutamate synthase (FPGS), which functions in one-carbon (C1) metabolism to polyglutamylate substrates of folate-dependent enzymes. Yeast complementation experiments demonstrated that expression of the maize bm4 gene in FPGS-deficient met7 yeast is able to rescue the yeast mutant phenotype, thus demonstrating that bm4 encodes a functional FPGS. Consistent with earlier studies, bm4 mutants exhibit a modest decrease in lignin concentration and an overall increase in the S:G lignin ratio relative to wild-type. Orthologs of bm4 include at least one paralogous gene in maize and various homologs in other grasses and dicots. Discovery of the gene underlying the bm4 maize phenotype illustrates a role for FPGS in lignin biosynthesis.
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