ArticleBMC immunology2014
Lipid raft-based membrane order is important for antigen-specific clonal expansion of CD4(+) T lymphocytes.
Article in BMC immunology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 25 citations in OpenAlex.
- The Role of 7-ketocholesterol in Placental Pathophysiology: Implications for Fetal Programming and Long-term Health: A Narrative Review.Reproductive sciences (Thousand Oaks, Calif.) · 2026Review
- Cholesterol in hematological malignancies: metabolism, mechanisms, and clinical implications.Frontiers in immunology · 2026Review
- How Membrane Phospholipids Containing Long-Chain Polyunsaturated Fatty Acids and Their Oxidation Products Orchestrate Lipid Raft Dynamics to Control Inflammation.The Journal of nutrition · 2024Review
- The ω-3 Polyunsaturated Fatty Acid Docosahexaenoic Acid Enhances NK-Cell Antitumor Effector Functions.Cancer immunology research · 2024Article
- Dysregulation of cellular membrane homeostasis as a crucial modulator of cancer risk.The FEBS journal · 2024Review
- Sitosterol-rich3 Biotech · 2023Article
- [Immunomodulation by nutritional intervention in critically ill patients].Die Anaesthesiologie · 2023Review
- CD4 and CD8 co-receptors modulate functional avidity of CD1b-restricted T cells.Nature communications · 2022Article
- Cholesterol-dependent plasma membrane order (LScientific reports · 2021Article
- Physical Activity and Nutritional Influence on Immune Function: An Important Strategy to Improve Immunity and Health Status.Frontiers in physiology · 2021Review
- Isolation of Lipid Rafts by the Detergent-Based and Non-detergent-Based Methods for Localization of GPCRs with Immunoblotting and Laser Scanning Confocal Microscopy.Methods in molecular biology (Clifton, N.J.) · 2021Article
- The Influence of Nutritional Factors on Immunological Outcomes.Frontiers in immunology · 2021Review
- Effects of Omega-3 Fatty Acids on Immune Cells.International journal of molecular sciences · 2019Review
- Functional link between plasma membrane spatiotemporal dynamics, cancer biology, and dietary membrane-altering agents.Cancer metastasis reviews · 2018Review
- Remodelling of primary human CD4+ T cell plasma membrane order by n-3 PUFA.The British journal of nutrition · 2018Article
- Engaging Ly-6A/Sca-1 triggers lipid raft-dependent and -independent responses in CD4Immunity, inflammation and disease · 2017Article
- Hypercholesterolemia Enhances T Cell Receptor Signaling and Increases the Regulatory T Cell Population.Scientific reports · 2017Article
- Glycerol Monolaurate (GML) inhibits human T cell signaling and function by disrupting lipid dynamics.Scientific reports · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLipid rafts are cholesterol and saturated lipid-rich, nanometer sized membrane domains that are hypothesized to play an important role in compartmentalization and spatiotemporal regulation of cellular signaling. Lipid rafts contribute to the plasma membrane order and to its spatial asymmetry, as well. The raft nanodomains on the surface of CD4(+) T lymphocytes coalesce during their interaction with antigen presenting cells (APCs). Sensing of foreign antigen by the antigen receptor on CD4(+) T cells occurs during these cell-cell interactions. In response to foreign antigen the CD4(+) T cells proliferate, allowing the expansion of few antigen-specific primary CD4(+) T cell clones. Proliferating CD4(+) T cells specialize in their function by undergoing differentiation into appropriate effectors tailored to mount an effective adaptive immune response against the invading pathogen.
resultsTo investigate the role of lipid raft-based membrane order in the clonal expansion phase of primary CD4(+) T cells, we have disrupted membrane order by incorporating an oxysterol, 7-ketocholesterol (7-KC), into the plasma membrane of primary CD4(+) T cells expressing a T cell receptor specific to chicken ovalbumin323-339 peptide sequence and tested their antigen-specific response. We report that 7-KC, at concentrations that disrupt lipid rafts, significantly diminish the c-Ovalbumin323-339 peptide-specific clonal expansion of primary CD4(+) T cells.
conclusionsOur findings suggest that lipid raft-based membrane order is important for clonal expansion of CD4(+) T cells in response to a model peptide.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.