ArticleACS nano2014
Second harmonic super-resolution microscopy for quantification of mRNA at single copy sensitivity.
Article in ACS nano, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed.
- Modern RNA Quantification Methods: From RT-qPCR to Advanced Microscopy.The journal of physical chemistry. B · 2026Review
- Myosin II tension sensors visualize force generation within the actin cytoskeleton in living cells.Journal of cell science · 2024Article
- Giant enhancement of optical nonlinearity from monolayer MoSNanophotonics (Berlin, Germany) · 2024Article
- Viewing life without labels under optical microscopes.Communications biology · 2023Review
- Organic Kainate Single Crystals for Second-Harmonic and Broadband THz Generation.ACS applied materials & interfaces · 2023Article
- Super-resolution re-scan second harmonic generation microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Article
- Characterization of fibrillar collagen isoforms in infarcted mouse hearts using second harmonic generation imaging.Biomedical optics express · 2021Article
- Antibody-Conjugated Barium Titanate Nanoparticles for Cell-Specific Targeting.ACS applied nano materials · 2020Article
- Super resolution measurement of collagen fibers in biological samples: Validation of a commercial solution for multiphoton microscopy.PloS one · 2020Article
- Vinculin Force Sensor Detects Tumor-Osteocyte Interactions.Scientific reports · 2019Article
- Crystalline heterogeneity in single ferroelectric nanocrystals revealed by polarized nonlinear microscopy.Scientific reports · 2019Article
- Improving resolution of second harmonic generation microscopy via scanning structured illumination.Biomedical optics express · 2018Article
- Superresolved multiphoton microscopy with spatial frequency-modulated imaging.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Multi-Order Investigation of the Nonlinear Susceptibility Tensors of Individual Nanoparticles.Scientific reports · 2016Article
- Optical Clearing Delivers Ultrasensitive Hyperspectral Dark-Field Imaging for Single-Cell Evaluation.ACS nano · 2016Article
- Single-Cell Quantification of Cytosine Modifications by Hyperspectral Dark-Field Imaging.ACS nano · 2015Article
- Single-cell screening and quantification of transcripts in cancer tissues by second-harmonic generation microscopy.Journal of biomedical optics · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Cell-specific information on the quantity and localization of key mRNAs at single copy sensitivity in single cells is critical for evaluating basic cellular process, disease risk, and efficacy of therapy. Quantification of overexpressed mRNAs beyond the diffraction limit is constrained by the optical property of the probes and microscopy techniques. In this report, nanosized barium titanium oxide (BaTiO3, BTO) crystals were utilized as probes for mRNA quantification by a second harmonic super-resolution microscopy (SHaSM). The SHaSM was able to detect a single copy of the human epidermal growth factor receptor 2 (Her2) mRNA at a resolution of 55.6 nm with the ability to resolve multiple mRNA copies in a diffraction-limited spot. Her2 mRNA per cell was counted in SK-BR-3, MCF-7, and HeLa cell lines as 595±79.1, 38.9±8.26, and 1.5±2.8, respectively. Our single-cell quantification results were validated with the fluorescence in situ hybridization studies and quantitative PCR, showing better specificity and selectivity over current single-molecule approaches for transcript detection. The SHaSM is expected to have an upper limit of resolving ∼10(4) transcripts in a single cell with the ability to monitor intracellular transcriptional dynamics at video rate. The developed approach has strong potential in clinical research and in the early diagnosis of life-threatening diseases such as cancer.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.