ArticleACS nano2014
Detection of two isomeric binding configurations in a protein-aptamer complex with a biological nanopore.
Article in ACS nano, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 65 citations in OpenAlex.
- Review
- Unlocking precision in aptamer engineering: a case study of the thrombin binding aptamer illustrates why modification size, quantity, and position matter.Nucleic acids research · 2024Article
- Review
- Mapping the conformational energy landscape of Abl kinase using ClyA nanopore tweezers.Nature communications · 2022Article
- Nanomaterial-Based Label-Free Electrochemical Aptasensors for the Detection of Thrombin.Biosensors · 2022Review
- Nanopore Technology for the Application of Protein Detection.Nanomaterials (Basel, Switzerland) · 2021Review
- A Biomimetic DNA-Based Membrane Gate for Protein-Controlled Transport of Cytotoxic Drugs.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2021Article
- A Biomimetic DNA-Based Membrane Gate for Protein-Controlled Transport of Cytotoxic Drugs.Angewandte Chemie (International ed. in English) · 2021Article
- Autonomous and Active Transport Operated by an Entropic DNA Piston.Nano letters · 2021Article
- Biological Nanopores: Engineering on Demand.Life (Basel, Switzerland) · 2021Review
- Sensing with Nanopores and Aptamers: A Way Forward.Sensors (Basel, Switzerland) · 2020Review
- Electro-Osmotic Vortices Promote the Capture of Folded Proteins by PlyAB Nanopores.Nano letters · 2020Article
- Directional conformer exchange in dihydrofolate reductase revealed by single-molecule nanopore recordings.Nature chemistry · 2020Article
- Engineering and Modeling the Electrophoretic Trapping of a Single Protein Inside a Nanopore.ACS nano · 2019Article
- A Nanopore Approach for Analysis of Caspase-7 Activity in Cell Lysates.Biophysical journal · 2019Article
- Energy Transfer as A Driving Force in Nucleic Acid⁻Protein Interactions.Molecules (Basel, Switzerland) · 2019Article
- Direct electrical quantification of glucose and asparagine from bodily fluids using nanopores.Nature communications · 2018Article
- The Utility of Nanopore Technology for Protein and Peptide Sensing.Proteomics · 2018Review
- Identification of single amino acid differences in uniformly charged homopolymeric peptides with aerolysin nanopore.Nature communications · 2018Article
- Real-Time Conformational Changes and Controlled Orientation of Native Proteins Inside a Protein Nanoreactor.Journal of the American Chemical Society · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 2 countries.
Funding
Abstract
Protein-DNA interactions play critical roles in biological systems, and they often involve complex mechanisms and dynamics that are not easily measured by ensemble experiments. Recently, we showed that folded proteins can be internalized inside ClyA nanopores and studied by ionic current recordings at the single-molecule level. Here, we use ClyA nanopores to sample the interaction between the G-quadruplex fold of the thrombin binding aptamer (TBA) and human thrombin (HT). Surprisingly, the internalization of the HT:TBA complex inside the nanopore induced two types of current blockades with distinguished residual current and lifetime. Using single nucleobase substitutions to TBA we showed that these two types of blockades originate from TBA binding to thrombin with two isomeric orientations. Voltage dependencies and the use of ClyA nanopores with two different diameters allowed assessing the effect of the applied potential and confinement and revealed that the two binding configurations of TBA to HT display different lifetimes. These results show that the ClyA nanopores can be used to probe conformational heterogeneity in protein:DNA interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.