ArticleCancer cell international2014
The immunoglobulin heavy chain VH6-1 promoter regulates Ig transcription in non-B cells.
Article in Cancer cell international, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 8 citations in OpenAlex.
- The Gene Rearrangement and Transcriptional Regulation of Non B Cell-Derived Immunoglobulin.Advances in experimental medicine and biology · 2024Review
- Staphylococcal Protein A Induces Leukocyte Necrosis by Complexing with Human Immunoglobulins.mBio · 2021Article
- Immunoglobulin Expression in Cancer Cells and Its Critical Roles in Tumorigenesis.Frontiers in immunology · 2021Review
- Expression of immunoglobulin G in human podocytes, and its role in cell viability and adhesion.International journal of molecular medicine · 2018Article
- IGK with conserved IGΚV/IGΚJ repertoire is expressed in acute myeloid leukemia and promotes leukemic cell migration.Oncotarget · 2015Article
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNon-B cell immunoglobulins (Igs) are widely expressed in epithelial cancer cells. The past 20 years of research have demonstrated that non-B cell Igs are associated with cancer cell proliferation, the cellular cytoskeleton and cancer stem cells. In this study we explored the transcriptional mechanism of IgM production in non-B cells.
methodsThe promoter region of a V-segment of the heavy mu chain gene (VH6-1) was cloned from a colon cancer cell line HT-29. Next, the promoter activities in non-B cells and B-cells were detected using the dual-luciferase reporter assay. Then the transcription factor binding to the promoter regions was evaluated by electrophoretic mobility shift assays (EMSAs) and gel supershift experiments.
resultsOur data showed that the sequence 1200 bp upstream of VH6-1 exhibited promoter activity in both B and non-B cells. No new regulatory elements were identified within the region 1200 bp to 300 bp upstream of VH6-1. In addition, Oct-1 was found to bind to the octamer element of the Ig gene promoter in cancer cells, in contrast to B cells, which utilize the transcriptional factor Oct-2.
conclusionThe regulatory mechanisms among different cell types controlling the production of IgM heavy chains are worth discussing.
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