ArticleProtein science : a publication of the Protein Society2015
Specific ion effects on macromolecular interactions in Escherichia coli extracts.
Article in Protein science : a publication of the Protein Society, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 37 citations in OpenAlex.
- Fine Tuning of the Arrangement of Non-Close-Packed Structures: Specific Ion Effects of Lanthanide Cations.ACS omega · 2026Article
- RNA Structure: Past, Future, and Gene Therapy Applications.International journal of molecular sciences · 2024Review
- Multivalent Calixarene Complexation of a Designed Pentameric Lectin.Biomacromolecules · 2024Article
- Radio Signals from Live Cells: The Coming of Age of In-Cell Solution NMR.Chemical reviews · 2022Review
- In-Cell Quantification of Drugs by Magic-Angle Spinning Dynamic Nuclear Polarization NMR.Journal of the American Chemical Society · 2022Article
- Connecting Longitudinal and Transverse Relaxation Rates in Live-Cell NMR.The journal of physical chemistry. B · 2020Article
- The Inescapable Effects of Ribosomes on In-Cell NMR Spectroscopy and the Implications for Regulation of Biological Activity.International journal of molecular sciences · 2019Review
- Interaction proteomics by using in-cell NMR spectroscopy.Journal of proteomics · 2019Review
- Real-Time In-Cell Nuclear Magnetic Resonance: Ribosome-Targeted Antibiotics Modulate Quinary Protein Interactions.Biochemistry · 2018Article
- Quantitative Determination of Interacting Protein Surfaces in Prokaryotes and Eukaryotes by Using In-Cell NMR Spectroscopy.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Viscosity Control of Protein Solution by Small Solutes: A Review.Current protein & peptide science · 2018Review
- Quinary interactions with an unfolded state ensemble.Protein science : a publication of the Protein Society · 2017Article
- Ribosome Mediated Quinary Interactions Modulate In-Cell Protein Activities.Biochemistry · 2017Article
- A cell is more than the sum of its (dilute) parts: A brief history of quinary structure.Protein science : a publication of the Protein Society · 2017Review
- In-cell NMR: a topical review.IUCrJ · 2017Review
- Protein charge determination and implications for interactions in cell extracts.Protein science : a publication of the Protein Society · 2017Article
- Total Cellular RNA Modulates Protein Activity.Biochemistry · 2016Article
- A Unique Tool for Cellular Structural Biology: In-cell NMR.The Journal of biological chemistry · 2016Review
- Intracellular pH modulates quinary structure.Protein science : a publication of the Protein Society · 2015Article
- Protein interaction patterns in different cellular environments are revealed by in-cell NMR.Scientific reports · 2015Article
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Authors and funding
4 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Protein characterization in situ remains a major challenge for protein science. Here, the interactions of ΔTat-GB1 in Escherichia coli cell extracts were investigated by NMR spectroscopy and size exclusion chromatography (SEC). ΔTat-GB1 was found to participate in high molecular weight complexes that remain intact at physiologically-relevant ionic strength. This observation helps to explain why ΔTat-GB1 was not detected by in-cell NMR spectroscopy. Extracts pre-treated with RNase A had a different SEC elution profile indicating that ΔTat-GB1 predominantly interacted with RNA. The roles of biological and laboratory ions in mediating macromolecular interactions were studied. Interestingly, the interactions of ΔTat-GB1 could be disrupted by biologically-relevant multivalent ions. The most effective shielding of interactions occurred in Mg(2+) -containing buffers. Moreover, a combination of RNA digestion and Mg(2+) greatly enhanced the NMR detection of ΔTat-GB1 in cell extracts.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.