ArticleBMC microbiology2014
Fragmentation of tRNA in Phytophthora infestans asexual life cycle stages and during host plant infection.
Article in BMC microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- A novel and ubiquitous miRNA-involved regulatory module ensures precise phosphorylation of RNA polymerase II and proper transcription.PLoS pathogens · 2024Article
- Multiple regulatory roles of the transfer RNA-derived small RNAs in cancers.Genes & diseases · 2024Review
- Distinct small RNAs are expressed at different stages ofFrontiers in genetics · 2024Article
- Identification of miRNAs Involving Potato-Plants (Basel, Switzerland) · 2023Article
- Review
- smartPARE: An R Package for Efficient Identification of True mRNA Cleavage Sites.International journal of molecular sciences · 2021Article
- Fusarium graminearum DICER-like-dependent sRNAs are required for the suppression of host immune genes and full virulence.PloS one · 2021Article
- The Evolutionary Significance of RNAi in the Fungal Kingdom.International journal of molecular sciences · 2020Review
- On the expanding roles of tRNA fragments in modulating cell behavior.Nucleic acids research · 2020Review
- Comparative transcriptome analysis shows the defense response networks regulated by miR482b.Plant cell reports · 2019Article
- Fine-Tuning of Gene Expression by tRNA-Derived Fragments during Abiotic Stress Signal Transduction.International journal of molecular sciences · 2018Review
- Computational Approaches to tRNA-Derived Small RNAs.Non-coding RNA · 2017Review
- Genome-wide identification and characterization of tRNA-derived RNA fragments in land plants.Plant molecular biology · 2017Article
- The 25-26 nt Small RNAs inFrontiers in microbiology · 2017Article
- The tRNA-Derived Small RNAs Regulate Gene Expression through Triggering Sequence-Specific Degradation of Target Transcripts in the Oomycete PathogenFrontiers in plant science · 2016Article
- Diverse Functions of Small RNAs in Different Plant-Pathogen Communications.Frontiers in microbiology · 2016Review
- tRNA-Derived Fragments (tRFs): Emerging New Roles for an Ancient RNA in the Regulation of Gene Expression.Life (Basel, Switzerland) · 2015Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundThe oomycete Phytophthora infestans possesses active RNA silencing pathways, which presumably enable this plant pathogen to control the large numbers of transposable elements present in its 240 Mb genome. Small RNAs (sRNAs), central molecules in RNA silencing, are known to also play key roles in this organism, notably in regulation of critical effector genes needed for infection of its potato host.
resultsTo identify additional classes of sRNAs in oomycetes, we mapped deep sequencing reads to transfer RNAs (tRNAs) thereby revealing the presence of 19-40 nt tRNA-derived RNA fragments (tRFs). Northern blot analysis identified abundant tRFs corresponding to half tRNA molecules. Some tRFs accumulated differentially during infection, as seen by examining sRNAs sequenced from P. infestans-potato interaction libraries. The putative connection between tRF biogenesis and the canonical RNA silencing pathways was investigated by employing hairpin RNA-mediated RNAi to silence the genes encoding P. infestans Argonaute (PiAgo) and Dicer (PiDcl) endoribonucleases. By sRNA sequencing we show that tRF accumulation is PiDcl1-independent, while Northern hybridizations detected reduced levels of specific tRNA-derived species in the PiAgo1 knockdown line.
conclusionsOur findings extend the sRNA diversity in oomycetes to include fragments derived from non-protein-coding RNA transcripts and identify tRFs with elevated levels during infection of potato by P. infestans.
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