ArticleGenetics2015
Rapid and precise engineering of the Caenorhabditis elegans genome with lethal mutation co-conversion and inactivation of NHEJ repair.
Article in Genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 149 papers.
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Who cites it
149 citing papers in PubMed.
- Article
- Engineering the Caenorhabditis elegans genome with a nested, self-excising selection cassette.Genetics · 2026Article
- Genome-wide analysis reveals pathways important for the development and maturation of excitatory synaptic connections to GABAergic neurons.G3 (Bethesda, Md.) · 2026Article
- The ubiquitin-proteasome pathway mediates selective degradation of unloaded Argonaute proteins in C. elegans.Genetics · 2026Article
- The Binding Landscape of an Essential Nuclear Hormone Receptor inbioRxiv : the preprint server for biology · 2025Article
- Niche-associated Type IV collagen promotes GLP-1/Notch receptor activation in the C. elegans germline.Nature communications · 2025Article
- Unraveling the functional dynamics of Caenorhabditis elegans stress-responsive omega class GST-44.The FEBS journal · 2025Article
- Induction of proline-rich proteins in response to tannin treatment in Caenorhabditis elegans.Scientific reports · 2025Article
- Efficient iterative CRISPR/Cas9 editing using sid-1 co-conversion and feeding RNAi in Caenorhabditis elegans.G3 (Bethesda, Md.) · 2025Article
- [Effect of DifferentSichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2025Article
- Multiscale patterning of a model apical extracellular matrix revealed by systematic endogenous protein tagging.bioRxiv : the preprint server for biology · 2025Article
- Rab GTPases are evolutionarily conserved signals mediating selective autophagy.The Journal of cell biology · 2025Article
- Non-cell-autonomous regulation of mTORC2 by Hedgehog signaling maintains lipid homeostasis.Cell reports · 2025Article
- A human progeria-associated BAF-1 mutation modulates gene expression and accelerates aging in C. elegans.The EMBO journal · 2024Article
- Dual tagging of GFP and Degron on endogenous COSA-1 inmicroPublication biology · 2024Article
- Lentiviral Transduction-based CRISPR/Cas9 Editing ofCurrent genomics · 2023Article
- A DEAD-box helicase drives the partitioning of a pro-differentiation NAB protein into nuclear foci.Nature communications · 2023Article
- Caenorhabditis elegans NSE3 homolog (MAGE-1) is involved in genome stability and acts in inter-sister recombination during meiosis.Genetics · 2023Article
- A simple method to dramatically increase C. elegans germline microinjection efficiency.Developmental biology · 2023Article
- Disparate roles for C. elegans DNA translocase paralogs RAD-54.L and RAD-54.B in meiotic prophase germ cells.Nucleic acids research · 2023Article
89 more citing papers are in PubMed but not listed here.
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Authors and funding
1 author.
Funding
Abstract
As in other organisms, CRISPR/Cas9 methods provide a powerful approach for genome editing in the nematode Caenorhabditis elegans. Oligonucleotides are excellent repair templates for introducing substitutions and short insertions, as they are cost effective, require no cloning, and appear in other organisms to target changes by homologous recombination at DNA double-strand breaks (DSBs). Here, I describe a methodology in C. elegans to efficiently knock in epitope tags in 8-9 days, using a temperature-sensitive lethal mutation in the pha-1 gene as a co-conversion marker. I demonstrate that 60mer oligos with 29 bp of homology drive efficient knock-in of point mutations, and that disabling nonhomologous end joining by RNAi inactivation of the cku-80 gene significantly improves knock-in efficiency. Homology arms of 35-80 bp are sufficient for efficient editing and DSBs up to 54 bp away from the insertion site produced knock-ins. These findings will likely be applicable for a range of genome editing approaches in C. elegans, which will improve editing efficiency and minimize screening efforts.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.