ArticleMolecular microbiology2015
Identification of the amino acids essential for LytSR-mediated signal transduction in Staphylococcus aureus and their roles in biofilm-specific gene expression.
Article in Molecular microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
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- First whole genome sequencing ofOpen veterinary journal · 2024Article
- Differential expression of small RNAs in biofilm-producing clinical methicillin-susceptibleHeliyon · 2024Article
- A Review of Biofilm Formation ofAntibiotics (Basel, Switzerland) · 2022Review
- Two-Component Systems ofGenes · 2021Review
- Synergistic effect of hypocrellin B and curcumin on photodynamic inactivation of Staphylococcus aureus.Microbial biotechnology · 2021Article
- Bacterial Protein Kinases.Advances in experimental medicine and biology · 2021Article
- Staphylococcal Biofilm Development: Structure, Regulation, and Treatment Strategies.Microbiology and molecular biology reviews : MMBR · 2020Review
- Low phosphatase activity of LiaS and strong LiaR-DNA affinity explain the unusual LiaS to LiaR in vivo stoichiometry.BMC microbiology · 2020Article
- CidR and CcpA Synergistically Regulate Staphylococcus aureusJournal of bacteriology · 2019Article
- Contribution of YjbIH to Virulence Factor Expression and Host Colonization inInfection and immunity · 2019Article
- Article
- Inactivation of the exogenous fatty acid utilization pathway leads to increased resistance to unsaturated fatty acids in Staphylococcus aureus.Microbiology (Reading, England) · 2019Article
- Urease is an essential component of the acid response network of Staphylococcus aureus and is required for a persistent murine kidney infection.PLoS pathogens · 2019Article
- Transcriptomic Adjustments ofFrontiers in microbiology · 2019Article
- Redirection of Metabolism in Response to Fatty Acid Kinase in Staphylococcus aureus.Journal of bacteriology · 2018Article
- Staphylococcus aureus biofilm: a complex developmental organism.Molecular microbiology · 2017Review
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8 authors.
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Abstract
Recent studies have demonstrated that expression of the Staphylococcus aureus lrgAB operon is specifically localized within tower structures during biofilm development. To gain a better understanding of the mechanisms underlying this spatial control of lrgAB expression, we carried out a detailed analysis of the LytSR two-component system. Specifically, a conserved aspartic acid (Asp53) of the LytR response regulator was shown to be the target of phosphorylation, which resulted in enhanced binding to the lrgAB promoter and activation of transcription. In addition, we identified His390 of the LytS histidine kinase as the site of autophosphorylation and Asn394 as a critical amino acid involved in phosphatase activity. Interestingly, LytS-independent activation of LytR was observed during planktonic growth, with acetyl phosphate acting as a phosphodonor to LytR. In contrast, mutations disrupting the function of LytS prevented tower-specific lrgAB expression, providing insight into the physiologic environment within these structures. In addition, overactivation of LytR led to increased lrgAB promoter activity during planktonic and biofilm growth and a change in biofilm morphology. Overall, the results of this study are the first to define the LytSR signal transduction pathway, as well as determine the metabolic context within biofilm tower structures that triggers these signaling events.
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