Evidence map›Paper›PMID 25491230›Full record

ArticleMedical science monitor : international medical journal of experimental and clinical research2014

New type of BACE1 siRNA delivery to cells.

Maciej Jabłkowski, Maciej Szemraj, Katarzyna Oszajca, Grażyna Janiszewska, Jacek Bartkowiak, Janusz Szemraj

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Medical science monitor : international medical journal of experimental and clinical research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 6 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Maciej JabłkowskiDepartment of Infectious and Liver Diseases, Medical University of Łódź, Łódź, Poland.
Maciej SzemrajDepartment of Medical Biochemistry, Medical University of Łódź, Łódź, Poland.
Katarzyna OszajcaDepartment of Medical Biochemistry, Medical University of Łódź, Łódź, Poland.
Grażyna JaniszewskaDepartment of Medical Biochemistry, Medical University of Łódź, Łódź, Poland.
Jacek BartkowiakDepartment of Medical Biochemistry, Medical University of Łódź, Łódź, Poland.
Janusz SzemrajDepartment of Medical Biochemistry, Medical University of Łódź, Łódź, Poland.
Medical University of Lodz · PL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundSmall interfering RNA (siRNA) gene therapy is a new molecular approach in the search for an efficient therapy for Alzheimer disease (AD), based on the principle of RNA interference. Reducing BACE activity can have great therapeutic potential for the treatment of AD. In this study, receptor-mediated delivery was used to deliver opioid peptide-conjugated BACE 1 to INR-32 human neuroblastoma cells. MATERIAL AND

methodsAn INR-32 human neuroblastoma cell line was stably transfected to express the APP cDNA coding fragment containing the predicted sites for cleavage by α, β, or γ-secretase. This was then treated with BACE 1 siRNA to silence BACE gene expression. BACE gene transcription and translation was determined using BACE-1 siRNA cross-linked with opioid peptide, together with RT-PCR, Western blot analysis, and ELISA.

resultsReceptor-mediated delivery was used to introduce BACE1 siRNA to the APP - INR 32 human neuroblastoma cells. Decreased BACE mRNA and protein expression were observed after the cells were transfected with BACE1 siRNA.

conclusionsDelivery of BACE1 siRNA appears to specifically reduce the cleavage of APP by inhibiting BACE1 activity.

Indexed as

Gene Transfer TechniquesAmyloid beta-Protein PrecursorAmyloid Precursor Protein SecretasesAspartic Acid EndopeptidasesBiological AssayBlotting, WesternCell Line, TumorGene Expression Regulation, NeoplasticGenetic VectorsHumansReceptors, OpioidRNA, MessengerRNA, Small InterferingTransfectionAmyloid beta-Protein PrecursorAmyloid Precursor Protein SecretasesAPP protein, humanAspartic Acid EndopeptidasesBACE1 protein, humanReceptors, OpioidRNA, MessengerRNA, Small Interfering

Identifiers

PMID25491230
PMCPMC4266366
OpenAlexW2149009427

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.