ArticleJournal of assisted reproduction and genetics2015
Improved blastocyst formation with reduced culture volume: comparison of three different culture conditions on 1128 sibling human zygotes.
Article in Journal of assisted reproduction and genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 37 citations in OpenAlex.
- Observational
- Index matching improves the imaging quality of 3D well-of-the-well dishes for embryo culture.Biophotonics discovery · 2026Article
- Non-invasive preimplantation genetic testing for aneuploidy using cell-free DNA in blastocyst culture medium.Journal of assisted reproduction and genetics · 2025Article
- Non-Invasive Preimplantation Genetic Testing.Genes · 2025Review
- Article
- Evolution of Minimally Invasive and Non-Invasive Preimplantation Genetic Testing: An Overview.Journal of clinical medicine · 2024Review
- Enhancement of preimplantation mouse embryo development with optimized in vitro culture dish via stabilization of medium osmolarity.Clinical and experimental reproductive medicine · 2023Article
- Considerations on staffing levels for a modern assisted reproductive laboratory.JBRA assisted reproduction · 2023Article
- The influence of day 3 embryo cell number on the clinical pregnancy and live birth rates of day 5 single blastocyst transfer from frozen embryo transfer cycles.BMC pregnancy and childbirth · 2022Article
- An Update on Non-invasive Approaches for Genetic Testing of the Preimplantation Embryo.Current genomics · 2022Review
- Embryo Density and Its Neutrality in Day-3 Embryo Development: A Retrospective Cohort Study.International journal of endocrinology · 2022Article
- Back to the future: optimised microwell culture of individual human preimplantation stage embryos.Journal of assisted reproduction and genetics · 2021Article
- Predictive value of the number of frozen blastocysts in live birth rates of the transferred fresh embryos.Journal of ovarian research · 2021Article
- Optimized culture systems for the preimplantation ICR mouse embryos with wide range of EDTA concentrations.Royal Society open science · 2021Article
- Reduced blastocyst formation in reduced culture volume.Journal of assisted reproduction and genetics · 2015Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe aim of the present randomized, comparative study was to evaluate the effect of reduced culture volumes on sibling human embryo development.
methodsFirstly, sibling injected oocytes obtained from 88 out of 165 consenting couples undergoing infertility treatment were cultured either in large (35 μl) or in small drops (15 μl) of culture medium. Secondly, sibling injected oocytes from 77 couples were cultured either in large (35 μl) or in mini drops (7 μl). Embryo quality on day-2 and day-3 and blastocyst formation rate on day-5 were evaluated.
resultsNo statistically significant difference in terms of embryo quality was detected comparing embryos cultured either in large (35 μl) or small (15 μl) drops until blastocyst stage. Similarly, no difference appeared between large (35 μl) or mini (7 μl) drops until day-3, however a significantly higher blastocyst formation rate was observed in mini (7 μl) drops on day-5.
conclusionsReduced culture volume seems not to influence early embryo development but a reduction of medium appears to positively affect blastocyst development. This supports the hypothesis that the pre-implantation embryo produces autocrine factors which exert a positive effect on embryo development when culture is performed in a reduced volume.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.