ArticleJournal of visualized experiments : JoVE2014
A guide to modern quantitative fluorescent western blotting with troubleshooting strategies.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed.
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- Technologies to investigate the mechanisms of force-based manipulations: a narrative methods review.Frontiers in pain research (Lausanne, Switzerland) · 2026Review
- Direct observation of fluorescent proteins in gels: A rapid, cost-efficient, and quantitative alternative to immunoblotting.Biology of the cell · 2025Article
- Preclinical evaluation of the theranostic potential ofEJNMMI radiopharmacy and chemistry · 2024Article
- A critical path to producing high quality, reproducible data from quantitative western blot experiments.Scientific reports · 2022Article
- An Optimized Comparative Proteomic Approach as a Tool in Neurodegenerative Disease Research.Cells · 2022Review
- ImmunoPET of CD146 in Orthotopic and Metastatic Breast Cancer Models.Bioconjugate chemistry · 2021Article
- A Comparative Analysis of Erythropoietin and Carbamoylated Erythropoietin Proteome Profiles.Life (Basel, Switzerland) · 2021Article
- Inhibiting IRE1α-endonuclease activity decreases tumor burden in a mouse model for hepatocellular carcinoma.eLife · 2020Article
- Bimodal size distribution immuno-quantum dots for fluorescent western blotting assay with high sensitivity and extended dynamic range.Mikrochimica acta · 2020Article
- Comparative proteomic profiling reveals mechanisms for early spinal cord vulnerability in CLN1 disease.Scientific reports · 2020Article
- Affinity-Bead Assisted Mass Spectrometry (Affi-BAMS): A Multiplexed Microarray Platform for Targeted Proteomics.International journal of molecular sciences · 2020Article
- In vivo study of gene expression with an enhanced dual-color fluorescent transcriptional timer.eLife · 2019Article
- A Western Blotting Protocol for Small Numbers of Hematopoietic Stem Cells.Journal of visualized experiments : JoVE · 2018Article
- Validating Antibodies for Quantitative Western Blot Measurements with Microwestern Array.Scientific reports · 2018Article
- Developmental and degenerative cardiac defects in the Taiwanese mouse model of severe spinal muscular atrophy.Journal of anatomy · 2018Article
- Reactive oxygen species stress increases accumulation of tyrosyl-DNA phsosphodiesterase 1 within mitochondria.Scientific reports · 2018Article
- MS Western, a Method of Multiplexed Absolute Protein Quantification is a Practical Alternative to Western Blotting.Molecular & cellular proteomics : MCP · 2018Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
The late 1970s saw the first publicly reported use of the western blot, a technique for assessing the presence and relative abundance of specific proteins within complex biological samples. Since then, western blotting methodology has become a common component of the molecular biologists experimental repertoire. A cursory search of PubMed using the term "western blot" suggests that in excess of two hundred and twenty thousand published manuscripts have made use of this technique by the year 2014. Importantly, the last ten years have seen technical imaging advances coupled with the development of sensitive fluorescent labels which have improved sensitivity and yielded even greater ranges of linear detection. The result is a now truly Quantifiable Fluorescence based Western Blot (QFWB) that allows biologists to carry out comparative expression analysis with greater sensitivity and accuracy than ever before. Many "optimized" western blotting methodologies exist and are utilized in different laboratories. These often prove difficult to implement due to the requirement of subtle but undocumented procedural amendments. This protocol provides a comprehensive description of an established and robust QFWB method, complete with troubleshooting strategies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.