ArticlePloS one2014
Transfection of Eimeria mitis with yellow fluorescent protein as reporter and the endogenous development of the transgenic parasite.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Genetic manipulation for the non-model protozoaniScience · 2025Review
- Developing efficient strategies for localizing the enhanced yellow fluorescent protein subcellularly in transgenic Eimeria parasites.Scientific reports · 2024Article
- Microneme-located VP2 inInfection and immunity · 2024Article
- Vaccines against chicken coccidiosis with particular reference to previous decade: progress, challenges, and opportunities.Parasitology research · 2022Review
- Establishment of RecombinantVaccines · 2021Article
- Towards Innovative Design and Application of RecombinantInfection and immunity · 2020Review
- Genetic modification of the protozoan Eimeria tenella using the CRISPR/Cas9 system.Veterinary research · 2020Article
- Efficient Single-Gene and Gene Family Editing in the Apicomplexan ParasiteFrontiers in bioengineering and biotechnology · 2020Article
- Investigating immune responses to parasites using transgenesis.Parasites & vectors · 2019Review
- TransgenicFrontiers in immunology · 2017Article
- Viral proteins expressed in the protozoan parasite Eimeria tenella are detected by the chicken immune system.Parasites & vectors · 2016Article
- "Self-cleaving" 2A peptide from porcine teschovirus-1 mediates cleavage of dual fluorescent proteins in transgenic Eimeria tenella.Veterinary research · 2016Article
- Chicken IgY Fc expressed by Eimeria mitis enhances the immunogenicity of E. mitis.Parasites & vectors · 2016Article
- Stable Transfection of Eimeria intestinalis and Investigation of Its Life Cycle, Reproduction and Immunogenicity.Frontiers in microbiology · 2016Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAdvancements have been made in the genetic manipulation of apicomplexan parasites. Both the in vitro transient and in vivo stable transfection of Eimeria tenella have been developed successfully. Herein, we report the transient and stable transfection of Eimeria mitis. METHODS AND
findingsSporozoites of E. mitis transfected with enhanced yellow fluorescent protein (EYFP) expression plasmid were inoculated into chickens via the cloacal route. The recovered fluorescent oocysts were sorted by fluorescence activated cell sorting (FACS) and then passaged 6 generations successively in chickens. The resulting population was analyzed by genome walking and Western blot. The endogenous development of the transgenic E. mitis was observed and its reproduction potential was tested. The stable transfection of E. mitis was developed. Genome walking confirmed the random integration of plasmid DNA into the genome; while Western blot analysis demonstrated the expression of foreign proteins. Constitutive expression of EYFP was observed in all stages of merogony, gametogony and sporogony. The peak of the transgenic oocyst output was delayed by 24 h and the total oocyst reproduction was reduced by 7-fold when compared to the parental strain.
conclusionStable transfection of E. mitis was successfully developed. The expression of foreign antigens in the transgenic parasites will facilitate the development of transgenic E. mitis as a vaccine vector.
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