ArticlePloS one2014
Transcriptomic immune response of the cotton stainer Dysdercus fasciatus to experimental elimination of vitamin-supplementing intestinal symbionts.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- Gut microbiota-derived butyrate primes systemic immunity in honey bees by mediating lipid metabolic reprogramming.Nature communications · 2026Article
- The species and abundance of gut bacteria both positively impact Phortica okadai behavior.Parasites & vectors · 2024Article
- Review
- Residual Effects of Transgenic Cotton on the Intestinal Microbiota ofMicroorganisms · 2023Article
- Transcriptome and metabolome analyses reveal the responses of brown planthoppers to RH resistant rice cultivar.Frontiers in physiology · 2022Article
- Influence ofInternational journal of molecular sciences · 2021Article
- Symbiont Genomic Features and Localization in the Bean BeetleApplied and environmental microbiology · 2021Article
- The Role of Bacterial Symbionts in Triatomines: An Evolutionary Perspective.Microorganisms · 2020Review
- Complex Relationships at the Intersection of Insect Gut Microbiomes and Plant Defenses.Journal of chemical ecology · 2020Review
- Transcriptomic and Metabolomic Analyses ofFrontiers in physiology · 2020Article
- Quantitative PCR primer design affects quantification of dsRNA-mediated gene knockdown.Ecology and evolution · 2019Article
- Established Cotton Stainer Gut Bacterial Mutualists Evade Regulation by Host Antimicrobial Peptides.Applied and environmental microbiology · 2019Article
- Symbiont transmission entails the risk of parasite infection.Biology letters · 2015Article
- Vitamin supplementation by gut symbionts ensures metabolic homeostasis in an insect host.Proceedings. Biological sciences · 2014Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The acquisition and vertical transmission of bacterial symbionts plays an important role in insect evolution and ecology. However, the molecular mechanisms underlying the stable maintenance and control of mutualistic bacteria remain poorly understood. The cotton stainer Dysdercus fasciatus harbours the actinobacterial symbionts Coriobacterium glomerans and Gordonibacter sp. in its midgut. The symbionts supplement limiting B vitamins and thereby significantly contribute to the host's fitness. In this study, we experimentally disrupted the symbionts' vertical transmission route and performed comparative transcriptomic analyses of genes expressed in the gut of aposymbiotic (symbiont-free) and control individuals to study the host immune response in presence and absence of the mutualists. Annotation of assembled cDNA reads identified a considerable number of genes involved in the innate immune system, including different protein isoforms of several immune effector proteins (specifically i-type lysozyme, defensin, hemiptericin, and pyrrhocoricin), suggesting the possibility for a highly differentiated response towards the complex resident microbial community. Gene expression analyses revealed a constitutive expression of transcripts involved in signal transduction of the main insect immune pathways, but differential expression of certain antimicrobial peptide genes. Specifically, qPCRs confirmed the significant down-regulation of c-type lysozyme and up-regulation of hemiptericin in aposymbiotic individuals. The high expression of c-type lysozyme in symbiont-containing bugs may serve to lyse symbiont cells and thereby harvest B-vitamins that are necessary for subsistence on the deficient diet of Malvales seeds. Our findings suggest a sophisticated host response to perturbation of the symbiotic gut microbiota, indicating that the innate immune system not only plays an important role in combating pathogens, but also serves as a communication interface between host and symbionts.
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