ArticleJournal of visualized experiments : JoVE2014
Specificity analysis of protein lysine methyltransferases using SPOT peptide arrays.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 36 citations in OpenAlex.
- Machine learning-driven prediction of substrates for enzymes introducing or removing protein post-translational modifications.Communications chemistry · 2025Article
- Mechanisms of Substrate Recognition by the Multispecific Protein Lysine Methyltransferase SETD6.Life (Basel, Switzerland) · 2025Article
- Investigation and design of the dual specificity of the PRDM9 protein lysine methyltransferase.Communications biology · 2025Article
- Orchestrating epigenetics: a comprehensive review of the methyltransferase SETD6.Experimental & molecular medicine · 2025Review
- SETDB1 activity is globally directed by H3K14 acetylation via its Triple Tudor Domain.Nucleic acids research · 2024Article
- Discovery of NSD2 non-histone substrates and design of a super-substrate.Communications biology · 2024Article
- Distinct specificities of the HEMK2 protein methyltransferase in methylation of glutamine and lysine residues.Protein science : a publication of the Protein Society · 2024Article
- Mapping of domain-mediated protein-protein interaction by SPOT peptide assay.STAR protocols · 2021Article
- The methyltransferase METTL9 mediates pervasive 1-methylhistidine modification in mammalian proteomes.Nature communications · 2021Article
- Mechanistic Insights into the Allosteric Regulation of the Clr4 Protein Lysine Methyltransferase by Autoinhibition and Automethylation.International journal of molecular sciences · 2020Article
- Analysis of the Substrate Specificity of the SMYD2 Protein Lysine Methyltransferase and Discovery of Novel Non-Histone Substrates.Chembiochem : a European journal of chemical biology · 2020Article
- Structural basis for the target specificity of actin histidine methyltransferase SETD3.Nature communications · 2019Article
- The dual methyltransferase METTL13 targets N terminus and Lys55 of eEF1A and modulates codon-specific translation rates.Nature communications · 2018Article
- Article
- Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation.Journal of visualized experiments : JoVE · 2017Article
- Analysis of Histone Antibody Specificity with Peptide Microarrays.Journal of visualized experiments : JoVE · 2017Article
- Arginine demethylation is catalysed by a subset of JmjC histone lysine demethylases.Nature communications · 2016Article
- Substrate Specificity of the HEMK2 Protein Glutamine Methyltransferase and Identification of Novel Substrates.The Journal of biological chemistry · 2016Article
- Histone H4 Lysine 20 (H4K20) Methylation, Expanding the Signaling Potential of the Proteome One Methyl Moiety at a Time.Molecular & cellular proteomics : MCP · 2016Review
- ArrayNinja: An Open Source Platform for Unified Planning and Analysis of Microarray Experiments.Methods in enzymology · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lysine methylation is an emerging post-translation modification and it has been identified on several histone and non-histone proteins, where it plays crucial roles in cell development and many diseases. Approximately 5,000 lysine methylation sites were identified on different proteins, which are set by few dozens of protein lysine methyltransferases. This suggests that each PKMT methylates multiple proteins, however till now only one or two substrates have been identified for several of these enzymes. To approach this problem, we have introduced peptide array based substrate specificity analyses of PKMTs. Peptide arrays are powerful tools to characterize the specificity of PKMTs because methylation of several substrates with different sequences can be tested on one array. We synthesized peptide arrays on cellulose membrane using an Intavis SPOT synthesizer and analyzed the specificity of various PKMTs. Based on the results, for several of these enzymes, novel substrates could be identified. For example, for NSD1 by employing peptide arrays, we showed that it methylates K44 of H4 instead of the reported H4K20 and in addition H1.5K168 is the highly preferred substrate over the previously known H3K36. Hence, peptide arrays are powerful tools to biochemically characterize the PKMTs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.