ArticlePloS one2014
Rapid identification and susceptibility testing of Candida spp. from positive blood cultures by combination of direct MALDI-TOF mass spectrometry and direct inoculation of Vitek 2.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- First Isolation of a Multi-azole-Resistant Aspergillus fumigatus cyp51A TRMycopathologia · 2025Article
- Direct Identification of Yeasts from Blood Cultures and Body Fluids Using MALDI-TOF MS with Concurrent Antifungal Susceptibility Testing.Mycopathologia · 2025Article
- Performance of Short-Term Culture and Direct MALDI-TOF MS for Identification of Candida Species From Blood Cultures.APMIS : acta pathologica, microbiologica, et immunologica Scandinavica · 2025Article
- Clinical evaluation of polymerase chain reaction coupled with quantum dot fluorescence analysis in the identification of bacteria and yeasts in patients with suspected bloodstream infections.Microbial biotechnology · 2023Article
- Fungal esophagitis associated with tuberculous pericarditis in an human immunodeficiency virus-positive patient: a case report.Journal of medical case reports · 2022Article
- Distribution Analysis ofJournal of microbiology and biotechnology · 2022Article
- Molecular phenotyping approaches for the detection and monitoring of carbapenem-resistant Enterobacteriaceae by mass spectrometry.Journal of mass spectrometry and advances in the clinical lab · 2022Review
- What IsMicroorganisms · 2022Review
- Rapid Simultaneous Testing of Multiple Antibiotics by the MALDI-TOF MS Direct-on-Target Microdroplet Growth Assay.Diagnostics (Basel, Switzerland) · 2021Article
- Direct Fluconazole Disk Susceptibility Testing for Candida glabrata-Positive Blood Cultures.Journal of clinical microbiology · 2021Article
- Direct Blood Culturing ofJournal of clinical microbiology · 2020Article
- Detection of Methicillin Resistance inFrontiers in microbiology · 2020Article
- A new culture-based method for rapid identification of microorganisms in polymicrobial blood cultures by MALDI-TOF MS.BMC microbiology · 2019Article
- ESICM/ESCMID task force on practical management of invasive candidiasis in critically ill patients.Intensive care medicine · 2019Article
- Fluorescent Capillary Electrophoresis Is Superior to Culture in DetectingJournal of clinical microbiology · 2019Article
- New Microbiological Techniques in the Diagnosis of Bloodstream Infections.Deutsches Arzteblatt international · 2018Review
- Evaluation of direct antifungal susceptibility testing methods of Candida spp. from positive blood culture bottles.Journal of clinical laboratory analysis · 2018Article
- Rapid and Accurate Identification of Candida albicans and Candida dubliniensis by Real-Time PCR and Melting Curve Analysis.Medical principles and practice : international journal of the Kuwait University, Health Science Centre · 2018Article
- An Automated Sample Preparation Instrument to Accelerate Positive Blood Cultures Microbial Identification by MALDI-TOF Mass Spectrometry (VitekFrontiers in microbiology · 2018Article
- Recent Advances and Ongoing Challenges in the Diagnosis of Microbial Infections by MALDI-TOF Mass Spectrometry.Frontiers in microbiology · 2018Article
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4 authors.
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Abstract
Fungaemia is associated with high mortality rates and early appropriate antifungal therapy is essential for patient management. However, classical diagnostic workflow takes up to several days due to the slow growth of yeasts. Therefore, an approach for direct species identification and direct antifungal susceptibility testing (AFST) without prior time-consuming sub-culturing of yeasts from positive blood cultures (BCs) is urgently needed. Yeast cell pellets prepared using Sepsityper kit were used for direct identification by MALDI-TOF mass spectrometry (MS) and for direct inoculation of Vitek 2 AST-YS07 card for AFST. For comparison, MALDI-TOF MS and Vitek 2 testing were performed from yeast subculture. A total of twenty four positive BCs including twelve C. glabrata, nine C. albicans, two C. dubliniensis and one C. krusei isolate were processed. Applying modified thresholds for species identification (score ≥ 1.5 with two identical consecutive propositions), 62.5% of BCs were identified by direct MALDI-TOF MS. AFST results were generated for 72.7% of BCs directly tested by Vitek 2 and for 100% of standardized suspensions from 24 h cultures. Thus, AFST comparison was possible for 70 isolate-antifungal combinations. Essential agreement (minimum inhibitory concentration difference ≤ 1 double dilution step) was 88.6%. Very major errors (VMEs) (false-susceptibility), major errors (false-resistance) and minor errors (false categorization involving intermediate result) amounted to 33.3% (of resistant isolates), 1.9% (of susceptible isolates) and 1.4% providing 90.0% categorical agreement. All VMEs were due to fluconazole or voriconazole. This direct method saved on average 23.5 h for identification and 15.1 h for AFST, compared to routine procedures. However, performance for azole susceptibility testing was suboptimal and testing from subculture remains indispensable to validate the direct finding.
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