ArticleProceedings of the National Academy of Sciences of the United States of America2014
Real-time monitoring of quorum sensing in 3D-printed bacterial aggregates using scanning electrochemical microscopy.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers.
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Who cites it
83 citing papers in PubMed.
- Taking Charge Against the Silent Pandemic-Electrochemical Characterization of Drug Resistance.Molecules (Basel, Switzerland) · 2026Review
- Mobile Protein Microboxes on Optical Fibers for Spatial Micromanipulation of Bacterial Microcolonies.Analytical chemistry · 2026Article
- Phytochemical Quorum-Sensing Inhibitors Against Bacterial Pathogens: Mechanisms of Action and Translational Challenges.Current issues in molecular biology · 2026Review
- Loss of Fsr quorum sensing promotes biofilm formation and worsens outcomes in enterococcal infective endocarditis.Nature communications · 2026Article
- Spatial structure: shaping the ecology and evolution of microbial communities.FEMS microbiology reviews · 2026Review
- Bacterial Species in Engineered Living Materials: Strategies and Future Directions.Microbial biotechnology · 2025Review
- Diffusible Signal Factors andBiology · 2025Review
- Use of analytical strategies to understand spatial chemical variation in bacterial surface communities.Journal of bacteriology · 2025Review
- Habitat fragmentation enhances microbial collective defence.Journal of the Royal Society, Interface · 2025Article
- Towards improved biofilm models.Nature reviews. Microbiology · 2025Review
- Targeting quorum sensing for manipulation of commensal microbiota.BMC biotechnology · 2024Review
- A 'rich-get-richer' mechanism drives patchy dynamics and resistance evolution in antibiotic-treated bacteria.Molecular systems biology · 2024Article
- CytochromeProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Nanoelectrochemistry at liquid/liquid interfaces for analytical, biological, and material applications.Chemical communications (Cambridge, England) · 2023Review
- A Brief Review of In Situ and Operando Electrochemical Analysis of Bacteria by Scanning Probes.Biosensors · 2023Review
- Periodically disturbing biofilms reduces expression of quorum sensing-regulated virulence factors iniScience · 2023Article
- Mechanistic Assessment of Metabolic Interaction between Single Oral Commensal Cells by Scanning Electrochemical Microscopy.Analytical chemistry · 2023Article
- Modeling Antibiotic Concentrations in the Vicinity of Antibiotic-Producing Bacteria at the Micron Scale.Applied and environmental microbiology · 2023Article
- Indirect Enrichment of Desirable, but Less Fit Phenotypes, from a Synthetic Microbial Community Using Microdroplet Confinement.ACS synthetic biology · 2023Article
- Charge Mapping ofAnalytical chemistry · 2023Article
23 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microbes frequently live in nature as small, densely packed aggregates containing ∼10(1)-10(5) cells. These aggregates not only display distinct phenotypes, including resistance to antibiotics, but also, serve as building blocks for larger biofilm communities. Aggregates within these larger communities display nonrandom spatial organization, and recent evidence indicates that this spatial organization is critical for fitness. Studying single aggregates as well as spatially organized aggregates remains challenging because of the technical difficulties associated with manipulating small populations. Micro-3D printing is a lithographic technique capable of creating aggregates in situ by printing protein-based walls around individual cells or small populations. This 3D-printing strategy can organize bacteria in complex arrangements to investigate how spatial and environmental parameters influence social behaviors. Here, we combined micro-3D printing and scanning electrochemical microscopy (SECM) to probe quorum sensing (QS)-mediated communication in the bacterium Pseudomonas aeruginosa. Our results reveal that QS-dependent behaviors are observed within aggregates as small as 500 cells; however, aggregates larger than 2,000 bacteria are required to stimulate QS in neighboring aggregates positioned 8 μm away. These studies provide a powerful system to analyze the impact of spatial organization and aggregate size on microbial behaviors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.