Evidence map›Paper›PMID 25488613›Full record

ArticleBlood cells, molecules & diseases2015

Dietary supplementation with docosahexanoic acid (DHA) increases red blood cell membrane flexibility in mice with sickle cell disease.

Nancy J Wandersee, Jamie L Maciaszek, Katie M Giger, Madelyn S Hanson, Suilan Zheng, YiHe Guo, Barbara Mickelson, Cheryl A Hillery, George Lykotrafitis, Philip S Low and 1 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Blood cells, molecules & diseases, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed, 1 pooled it
0.8field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 1 synthesis or guideline pooled it, 34 citations in OpenAlex.

  1. Pooled it
  2. Trial
  3. Review
  4. Article
  5. Article
  6. Article
  7. Article
  8. SC411 treatment can enhance survival in a mouse model of sickle cell disease.Prostaglandins, leukotrienes, and essential fatty acids · 2020
    Article
  9. Article
  10. Article
  11. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 1 country.

Nancy J WanderseeDepartment of Pediatrics, Medical College of Wisconsin, Milwaukee, WI, USA; Children's Research Institute, Medical College of Wisconsin, Milwaukee, WI, USA; Blood Research Institute, Blood Center of Wisconsin, Milwaukee, WI, USA. Electronic address: nancy.wandersee@bcw.edu.
Jamie L MaciaszekDepartment of Mechanical Engineering, University of Connecticut, Storrs, CT, USA.
Katie M GigerDepartment of Chemistry, Purdue University, West Lafayette, IN, USA.
Madelyn S HansonBlood Research Institute, Blood Center of Wisconsin, Milwaukee, WI, USA; Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI, USA.
Suilan ZhengDepartment of Chemistry, Purdue University, West Lafayette, IN, USA.
YiHe GuoDepartment of Pediatrics, Medical College of Wisconsin, Milwaukee, WI, USA; Children's Research Institute, Medical College of Wisconsin, Milwaukee, WI, USA; Blood Research Institute, Blood Center of Wisconsin, Milwaukee, WI, USA.
Barbara MickelsonTechnical Services, Harlan-Teklad Laboratories, Inc., Madison, WI, USA.
Cheryl A HilleryDepartment of Pediatrics, Medical College of Wisconsin, Milwaukee, WI, USA; Children's Research Institute, Medical College of Wisconsin, Milwaukee, WI, USA; Blood Research Institute, Blood Center of Wisconsin, Milwaukee, WI, USA.
George LykotrafitisDepartment of Mechanical Engineering, University of Connecticut, Storrs, CT, USA.
Philip S LowDepartment of Chemistry, Purdue University, West Lafayette, IN, USA.
Neil HoggDepartment of Biophysics, Medical College of Wisconsin, Milwaukee, WI, USA.
Medical College of Wisconsin · USPurdue University West Lafayette · USChildren's Hospital of Wisconsin · USUniversity of Connecticut · US

Funding

Nociceptive Mechanisms Underlying Sickle Cell PainR01NS070711 · NINDS · MEDICAL COLLEGE OF WISCONSIN · PI Bonnie N Dittel, Cheryl Louise Stucky · 2009 to 2026
$8.7M
Studies of Erythrocyte Membrane StructureR01GM024417 · NIGMS · PURDUE UNIVERSITY WEST LAFAYETTE · PI LOW, PHILIP STEWART · 1985 to 2020
$5.4M
Mechanisms of Inflammation in Sickle Cell DiseaseR01HL128371 · NHLBI · MEDICAL COLLEGE OF WISCONSIN · PI PRITCHARD, KIRKWOOD ARTHUR · 2016 to 2024
$4.2M
ERYTHROCYTE MEMBRANE STRUCTURER37GM024417 · NIGMS · PURDUE UNIVERSITY WEST LAFAYETTE · PI LOW, PHILIP STEWART · 1999 to 2008
$3.5M
Sickle Cell ScholarU54HL090503 · NHLBI · MEDICAL COLLEGE OF WISCONSIN · PI PANEPINTO, JULIE A · 2008 to 2011
$3.0M
RESEARCH TRAINING IN TRANSFUSION SCIENCET32HL007209 · NHLBI · VERSITI WISCONSIN, INC. · PI WHITE, II, GILBERT C. · 1985 to 2019
$2.9M
Asthma Increases Vaso-occlusion in Sickle Cell DiseaseR01HL102836 · NHLBI · MEDICAL COLLEGE OF WISCONSIN · PI HILLERY, CHERYL A, PRITCHARD, KIRKWOOD ARTHUR · 2010 to 2013
$2.2M
NHLBI NIH HHS 5T32HL007209-33NHLBI NIH HHS HL090503NHLBI NIH HHS HL102836NHLBI NIH HHS R01 HL102836NHLBI NIH HHS R01 HL128371NHLBI NIH HHS T32 HL007209NHLBI NIH HHS U54 HL090503NIGMS NIH HHS GM24417-34NIGMS NIH HHS R01 GM024417NIGMS NIH HHS R37 GM024417NINDS NIH HHS NS070711NINDS NIH HHS R01 NS070711
6 · The paper itself

Abstract

Humans and mice with sickle cell disease (SCD) have rigid red blood cells (RBCs). Omega-3 fatty acids, such as docosahexanoic acid (DHA), may influence RBC deformability via incorporation into the RBC membrane. In this study, sickle cell (SS) mice were fed natural ingredient rodent diets supplemented with 3% DHA (DHA diet) or a control diet matched in total fat (CTRL diet). After 8weeks of feeding, we examined the RBCs for: 1) stiffness, as measured by atomic force microscopy; 2) deformability, as measured by ektacytometry; and 3) percent irreversibly sickled RBCs on peripheral blood smears. Using atomic force microscopy, it is found that stiffness is increased and deformability decreased in RBCs from SS mice fed CTRL diet compared to wild-type mice. In contrast, RBCs from SS mice fed DHA diet had markedly decreased stiffness and increased deformability compared to RBCs from SS mice fed CTRL diet. Furthermore, examination of peripheral blood smears revealed less irreversibly sickled RBCs in SS mice fed DHA diet as compared to CTRL diet. In summary, our findings indicate that DHA supplementation improves RBC flexibility and reduces irreversibly sickled cells by 40% in SS mice. These results point to potential therapeutic benefits of dietary omega-3 fatty acids in SCD.

Indexed as

Dietary SupplementsAnemia, Sickle CellAnimalsDisease Models, AnimalDocosahexaenoic AcidsErythrocyte CountErythrocyte DeformabilityErythrocyte MembraneHumansMiceMice, Inbred C57BLMicroscopy, Atomic ForceDocosahexaenoic AcidsAnimal modelOmega-3Red blood cell disordersRed cell membraneSickle cell anemia

Identifiers

PMID25488613
PMCPMC4297559
OpenAlexW2081614460

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.