ArticlePlant molecular biology2015
Functional analysis of OsPGIP1 in rice sheath blight resistance.
Article in Plant molecular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 1 synthesis or guideline pooled it, 69 citations in OpenAlex.
- Pooled it
- AI-Integrated Omics Analysis Reveals Cultivar-Specific Resistance Mechanisms to Powdery Mildew inInternational journal of molecular sciences · 2025Article
- Advances in breeding, biotechnology, and nanotechnological approaches to combat sheath blight disease in rice.Molecular biology reports · 2024Review
- Comparative transcriptome analysis of rice cultivars resistant and susceptible to Rhizoctonia solani AG1-IA.BMC genomics · 2022Article
- Gene expression analysis of resistant and susceptible rice cultivars to sheath blight after inoculation with Rhizoctonia solani.BMC genomics · 2022Article
- Comparative transcriptome profiling reveals the basis of differential sheath blight disease response in tolerant and susceptible rice genotypes.Protoplasma · 2022Article
- HighFrontiers in plant science · 2022Article
- Cytological observation and transcriptome analysis reveal dynamic changes ofFrontiers in plant science · 2022Article
- Comprehensive analysis of peptide-coding genes and initial characterization of an LRR-only microprotein inFrontiers in plant science · 2022Article
- Loose Plant Architecture 1-Interacting Kinesin-like Protein KLP Promotes Rice Resistance to Sheath Blight Disease.Rice (New York, N.Y.) · 2021Article
- Strategies to Manage Rice Sheath Blight: Lessons from Interactions between Rice and Rhizoctonia solani.Rice (New York, N.Y.) · 2021Review
- Article
- Transcriptome analysis of different rice cultivars provides novel insights into the rice response to bacterial leaf streak infection.Functional & integrative genomics · 2020Article
- Direct evidence for a new mode of plant defense against insects via a novel polygalacturonase-inhibiting protein expression strategy.The Journal of biological chemistry · 2020Article
- Understanding sheath blight resistance in rice: the road behind and the road ahead.Plant biotechnology journal · 2020Review
- Indeterminate Domain Proteins Regulate Rice Defense to Sheath Blight Disease.Rice (New York, N.Y.) · 2020Article
- Comprehensive construction strategy of bidirectional green tissue-specific synthetic promoters.Plant biotechnology journal · 2020Article
- OsPGIP1-Mediated Resistance to Bacterial Leaf Streak in Rice is Beyond Responsive to the Polygalacturonase of Xanthomonas oryzae pv. oryzicola.Rice (New York, N.Y.) · 2019Article
- Review
- Amino acid substitutions in a polygalacturonase inhibiting protein (OsPGIP2) increases sheath blight resistance in rice.Rice (New York, N.Y.) · 2019Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As one of the most devastating diseases of rice, sheath blight causes severe rice yield loss. However, little progress has been made in rice breeding for sheath blight resistance. It has been reported that polygalacturonase inhibiting proteins can inhibit the degradation of the plant cell wall by polygalacturonases from pathogens. Here, we prokaryotically expressed and purified OsPGIP1 protein, which was verified by Western blot analysis. Activity assay confirmed the inhibitory activity of OsPGIP1 against the PGase from Rhizoctonia solani. In addition, the location of OsPGIP1 was determined by subcellular localization. Subsequently, we overexpressed OsPGIP1 in Zhonghua 11 (Oryza sativa L. ssp. japonica), and applied PCR and Southern blot analysis to identify the positive T0 transgenic plants with single-copy insertions. Germination assay of the seeds from T1 transgenic plants was carried out to select homozygous OsPGIP1 transgenic lines, and the expression levels of OsPGIP1 in these lines were analyzed by quantitative real-time PCR. Field testing of R. solani inoculation showed that the sheath blight resistance of the transgenic rice was significantly improved. Furthermore, the levels of sheath blight resistance were in accordance with the expression levels of OsPGIP1 in the transgenic lines. Our results reveal the functions of OsPGIP1 and its resistance mechanism to rice sheath blight, which will facilitate rice breeding for sheath blight resistance.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.