ArticleBMC biology2014
Generation of comprehensive transposon insertion mutant library for the model archaeon, Haloferax volcanii, and its use for gene discovery.
Article in BMC biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 30 citations in OpenAlex.
- Uncovering transcriptional processes in microbial communities adapted to differing saline conditions in salt-weathered historic buildings.Microbiome · 2026Article
- A temperature-sensitive mutant screen reveals a translational stress-induced cell-cycle arrest in a thermophilic archaeon.Molecular biology of the cell · 2025Article
- Review
- Identification of structural and regulatory cell-shape determinants in Haloferax volcanii.Nature communications · 2024Article
- Dissecting the Arginine and Lysine Biosynthetic Pathways and Their Relationship in Haloarchaeon Natrinema gari J7-2 via Endogenous CRISPR-Cas System-Based Genome Editing.Microbiology spectrum · 2023Article
- The Best of Both Worlds: Discovery-Driven Learning through a Lab-Seminar Approach.Journal of microbiology & biology education · 2022Article
- Transposon Insertion Mutagenesis in Hyperthermophilic Crenarchaeon Sulfolobus islandicus.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Open Issues for Protein Function Assignment inGenes · 2021Article
- Article
- Review
- Global Transcriptional Programs in Archaea Share Features with the Eukaryotic Environmental Stress Response.Journal of molecular biology · 2019Review
- Limited Cross-Complementation BetweenFrontiers in microbiology · 2019Article
- Molecular Factors of Hypochlorite Tolerance in the Hypersaline Archaeon Haloferax volcanii.Genes · 2018Article
- Mu transpososome activity-profiling yields hyperactive MuA variants for highly efficient genetic and genome engineering.Nucleic acids research · 2018Article
- High tolerance to self-targeting of the genome by the endogenous CRISPR-Cas system in an archaeon.Nucleic acids research · 2017Article
- Screening of a Haloferax volcanii Transposon Library Reveals Novel Motility and Adhesion Mutants.Life (Basel, Switzerland) · 2016Article
- Random mutagenesis of the hyperthermophilic archaeon Pyrococcus furiosus using in vitro mariner transposition and natural transformation.Scientific reports · 2016Article
- A manual curation strategy to improve genome annotation: application to a set of haloarchael genomes.Life (Basel, Switzerland) · 2015Article
- Archaeal type IV pili and their involvement in biofilm formation.Frontiers in microbiology · 2015Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundArchaea share fundamental properties with bacteria and eukaryotes. Yet, they also possess unique attributes, which largely remain poorly characterized. Haloferax volcanii is an aerobic, moderately halophilic archaeon that can be grown in defined media. It serves as an excellent archaeal model organism to study the molecular mechanisms of biological processes and cellular responses to changes in the environment. Studies on haloarchaea have been impeded by the lack of efficient genetic screens that would facilitate the identification of protein functions and respective metabolic pathways.
resultsHere, we devised an insertion mutagenesis strategy that combined Mu in vitro DNA transposition and homologous-recombination-based gene targeting in H. volcanii. We generated an insertion mutant library, in which the clones contained a single genomic insertion. From the library, we isolated pigmentation-defective and auxotrophic mutants, and the respective insertions pinpointed a number of genes previously known to be involved in carotenoid and amino acid biosynthesis pathways, thus validating the performance of the methodologies used. We also identified mutants that had a transposon insertion in a gene encoding a protein of unknown or putative function, demonstrating that novel roles for non-annotated genes could be assigned.
conclusionsWe have generated, for the first time, a random genomic insertion mutant library for a halophilic archaeon and used it for efficient gene discovery. The library will facilitate the identification of non-essential genes behind any specific biochemical pathway. It represents a significant step towards achieving a more complete understanding of the unique characteristics of halophilic archaea.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.